Missing Amide I Mode in Gap-Mode Tip-Enhanced Raman Spectra of Proteins

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Author(s) Blum, Carolin, Schmid, Thomas, Opilik, Lothar, Metanis, Norman, Weidmann, Simon, Zenobi, Renato
Publication Type Journal Items, Publication Status: Published
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Title Missing Amide I Mode in Gap-Mode Tip-Enhanced Raman Spectra of Proteins
Author(s) Blum, Carolin
Schmid, Thomas
Opilik, Lothar
Metanis, Norman
Weidmann, Simon
Zenobi, Renato
Journal or Series Title The journal of physical chemistry. C, Energy conversion and storage, optical and electronic devices, interfaces, nanomaterials, and hard matter
Volume Number 116
Issue Number 43
Start Page 23061
End Page 23066
ISSN 1932-7447
1932-7455
Publisher American Chemical Society
Publication Place Washington, DC
Publication Date 2012
Abstract Tip-enhanced Raman spectroscopy is a surface sensitive analytical method that combines the advantages of scanning probe microscopy and Raman spectroscopy. It holds great promises for imaging of biological samples with high spatial resolution (10-50 nm), well below the optical diffraction limit. It offers the opportunity to directly localize and identify proteins and their conformation in a complex (e.g., native) environment. Tip-enhanced Raman.(TER) spectra in the so-called "gap-mode" configuration with a metal tip in scanning tunnelling feedback with a metal substrate coated with different proteins (bovine serum albumin, immunoglobulin G, trypsin, and beta-lactoglobulin) as well as of model octapeptides (with and without an aromatic amino acid residue) are presented. The goal was to determine if it is possible to reliably assign marker bands for proteins and if different secondary structures of proteins can be distinguished in their gap-mode TER spectra as reliably as by IR and conventional Raman spectroscopy. It is shown that contrary to the presented conventional Raman spectra of proteins the amide I mode, which is widely used to identify secondary structure motifs of proteins, is not visible in gap-mode TEAS. Aromatic modes are prominent and can be used as reliable marker bands for imaging of proteins in a complex environment.
DOI 10.1021/jp306831p
Additional Notes Received 10 July 2012, Revised 27 September 2012
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03430
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NEBIS System Number 005283485
Source Database ID WOS-000310482900052
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@article{Blm2012,
  author = "Blum, Carolin and Schmid, Thomas and Opilik, Lothar and Metanis, Norman and Weidmann, Simon and Zenobi, Renato",
  title = "{M}issing {A}mide {I} {M}ode in {G}ap-{M}ode {T}ip-{E}nhanced {R}aman {S}pectra of {P}roteins",
  journal = "The journal of physical chemistry. C, Energy conversion and storage, optical and electronic devices, interfaces, nanomaterials, and hard matter",
  year = 2012,
  volume = "116",
  number = "43",
  pages = "23061--23066",
}


E-Citations record created: Thu, 03 Jan 2013, 08:15:53 CET