Ionic liquids as matrices in microfluidic sample deposition for high-mass matrix-assisted laser desorption/ionization mass spectrometry

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Author(s) Weidmann, Simon, Kemmerling, Simon, Mädler, Stefanie, Stahlberg, Henning, Braun, Thomas, Zenobi, Renato
Publication Type Journal Items, Publication Status: Published
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Title Ionic liquids as matrices in microfluidic sample deposition for high-mass matrix-assisted laser desorption/ionization mass spectrometry
Author(s) Weidmann, Simon
Kemmerling, Simon
Mädler, Stefanie
Stahlberg, Henning
Braun, Thomas
Zenobi, Renato
Journal or Series Title European journal of mass spectrometry
Volume Number 18
Issue Number 3
Start Page 279
End Page 286
ISSN 1751-6838
1469-0667
1356-1049
Publisher IM Publications
Publication Place Chichester
Publication Date 2012
Keyword(s) MALDI mass spectrometry
Ionic Liquids
High-mass protein analysis
Microfluidic sample preparation
Abstract Sample preparation for matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) via a microfluidic deposition device using ionic liquid matrices addresses several problems of standard protocols with crystalline matrices, such as the heterogeneity of sample spots due to the co-crystallization of sample and matrix and the limited capability for high-throughput analysis. Since ionic Liquid matrices do not solidify during the measurement, the resulting sample spots are homogeneous. The use of these matrices is also beneficial for automated sample preparation, since crystallization of the matrix is avoided and, thus, no clogging of the spotting device can occur. The applicability of ionic liquids to the analysis of biomolecules with high molecular weights, up to approximate to 1 MDa is shown, as well as a good sensitivity [5fmol] for recombinant human fibronectin, a protein with a molecular weight of 226 kDa. Microfluidic sample deposition of proteins with high molecular weights will, in the future, allow parallel sample preparation for MALDI-MS and for electron microscopy.
DOI 10.1255/ejms.1182
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03430
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@article{Wdmnn2012,
  author = "Weidmann, Simon and Kemmerling, Simon and M{\"{a}}dler, Stefanie and Stahlberg, Henning and Braun, Thomas and Zenobi, Renato",
  title = "{I}onic liquids as matrices in microfluidic sample deposition for high-mass matrix-assisted laser desorption/ionization mass spectrometry",
  journal = "European journal of mass spectrometry",
  year = 2012,
  volume = "18",
  number = "3",
  pages = "279--286",
}


E-Citations record created: Mon, 29 Oct 2012, 10:02:21 CET