Open access
Date
2012-08-22Type
- Journal Article
ETH Bibliography
yes
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Abstract
Acid production from rhamnose is a characteristic phenotype of Listeria monocytogenes. We report the identification of the rhamnose transport and utilization operon located at lmo2846 to lmo2851, including the rhamnose-dependent promoter Prha. Expression of reporter genes under control of Prha on a single copy integration vector demonstrated its suitability for inducible gene expression in L. monocytogenes. Transcription initiation from Prha is dose dependent, and a concentration as low as 100 µM rhamnose was found sufficient for induction. Moreover, Prha is subject to glucose catabolite repression, which provides additional options for strict control of expression. Infection of human THP1 macrophages revealed that Prha is repressed in intracellular L. monocytogenes, which is explained by the absence of rhamnose in the cytosol and possible interference by catabolite repression. The Prha promoter provides a novel and useful tool for triggering gene expression in extracellular L. monocytogenes, whereas intracellular conditions prevent transcription from this promoter. Show more
Permanent link
https://doi.org/10.3929/ethz-b-000056241Publication status
publishedExternal links
Journal / series
PLoS ONEVolume
Pages / Article No.
Publisher
PLOSOrganisational unit
03651 - Loessner, Martin / Loessner, Martin
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ETH Bibliography
yes
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