Mass spectrometric determination of association constants of adenylate kinase with two noncovalent inhibitors

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Author(s) Daniel, Jürg M., McCombie, Gregor, Wendt, Silke, Zenobi, Renato
Publication Type Journal Items, Publication Status: Published
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Title Mass spectrometric determination of association constants of adenylate kinase with two noncovalent inhibitors
Author(s) Daniel, Jürg M.
McCombie, Gregor
Wendt, Silke
Zenobi, Renato
Journal or Series Title Journal of the American Society for Mass Spectrometry
Volume Number 14
Issue Number 5
Start Page 442
End Page 448
ISSN 1044-0305
1879-1123
Publisher Springer
Publication Place New York
Publication Date 2003-05
Abstract Noncovalent complexes between chicken muscle adenylate kinase and two inhibitors, P-1, P-4- di(adenosine-5')tetraphosphate (Ap4A) and P-1,P-5-di(adenosine-5') pentaphosphate (Ap5A), were investigated with electrospray ionization mass spectrometry under non-denaturing conditions. The nonconvalent nature and the specificity of the complexes are demonstrated with a number of control experiments. Titration experiments allowed the association constants for inhibitor binding to be determined. Problems with concentration dependent ion yields are circumvented by a data evaluation method that is insensitive to the overall ionization efficiency. The K-a values found were 9.0 X 10(4) M-1 (Ap4A) and 4.0 X 10(7) M-1 (Ap5A), respectively, in very good agreement with available literature data.
DOI 10.1016/S1044-0305(03)00132-6
Additional Notes Published online 4 April 2003
Document Type Article
Publication Status Published
Language English
NEBIS System Number 000541631
Source Database ID WOS-000183169400004
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@article{Dnl2003,
  author = "Daniel, J{\"{u}}rg M. and McCombie, Gregor and Wendt, Silke and Zenobi, Renato",
  title = "{M}ass spectrometric determination of association constants of adenylate kinase with two noncovalent inhibitors",
  journal = "Journal of the American Society for Mass Spectrometry",
  year = 2003,
  volume = "14",
  number = "5",
  pages = "442--448",
  month = may,
}


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