Mass spectrometric studies of dissociation constants of noncovalent complexes

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Author(s) Boeri Erba, Elisabetta, Zenobi, Renato
Publication Type Journal Items, Publication Status: Published
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Title Mass spectrometric studies of dissociation constants of noncovalent complexes
Author(s) Boeri Erba, Elisabetta
Zenobi, Renato
Journal or Series Title Annual reports on the progress of chemistry. Section C, Physical chemistry
Volume Number 107
Start Page 199
End Page 228
ISSN 0260-1826
1460-4787
Publisher Royal Society of Chemistry
Publication Place Cambridge
Publication Date 2011
Abstract Specific interactions among biomolecules to form noncovalently bound complexes play a pivotal role in key cellular processes such as cell division, cell signalling, gene transcription and translation. The propensity of noncovalently bound complexes to dissociate into their components can be quantified and constants of dissociation (Kd) can be obtained by various methods. Mass spectrometry has become an important method to measure Kd. The advent of soft ionisation techniques, in particular electrospray ionisation (ESI) and matrix-assisted laser desorption/ionisation (MALDI) has established mass spectrometry as a viable technique for investigating noncovalent interactions and for quantifying their binding strengths. Under carefully chosen experimental and instrumental conditions, it is possible to observe intact noncovalent complexes in the gas phase using ESI and MALDI, and to use the mass spectra as a read-out for determining solution-phase Kd. Compared to other biophysical methods, mass spectrometry is highly sensitive and fast, and gives additional information about the stoichiometry and specificity of noncovalent interactions. This review focuses on recent MS-based methodologies for quantification of binding strengths, in particular those that promise to complement conventional biophysical methods.
DOI 10.1039/C1PC90006D
Additional Notes First published online 18 April 2011
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03430
Organisational Unit(s)
NEBIS System Number 000039015
Source Database ID FORM-1326275509
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@article{Br2011,
  author = "Boeri Erba, Elisabetta and Zenobi, Renato",
  title = "{M}ass spectrometric studies of dissociation constants of noncovalent complexes",
  journal = "Annual reports on the progress of chemistry. Section C, Physical chemistry",
  year = 2011,
  volume = "107",
  pages = "199--228",
}


E-Citations record created: Wed, 11 Jan 2012, 09:52:08 CET