The Role of Nebulizer Gas Flow in Electrosonic Spray Ionization (ESSI)

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Author(s) Wang, Rui, Allmendinger, Pitt, Zhu, Liang, Groehn, Arto Juhani, Wegner, Karsten, Frankevich, Vladimir, Zenobi, Renato
Publication Type Journal Items, Publication Status: Published
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Title The Role of Nebulizer Gas Flow in Electrosonic Spray Ionization (ESSI)
Author(s) Wang, Rui
Allmendinger, Pitt
Zhu, Liang
Groehn, Arto Juhani
Wegner, Karsten
Frankevich, Vladimir
Zenobi, Renato
Journal or Series Title Journal of the American Society for Mass Spectrometry
Volume Number 22
Issue Number 7
Start Page 1234
End Page 1241
ISSN 1044-0305
Publisher Springer
Publication Place New York, NY, USA
Publication Date 2011-07
Keyword(s) Electrosonic spray ionization
Electrospray ionization
Ionization mechanism
Abstract In this work, we investigated the role of the nebulizer gas flow in electrosonic spray ionization (ESSI), by systematically studying the relation between the flow and the ion signals of proteins, such as cytochrome c and holomyoglobin using ESSI-mass spectrometry (MS). When a neutral solution was delivered with a small sample flow rate (a parts per thousand currency sign5 mu L/min), no obvious transition from electrospray ionization (ESI) to ESSI was found as the gas velocity varies from subsonic to supersonic speed. Droplets mostly experienced acceleration instead of breakup by the high-speed nebulizer gas. On the contrary, using particular experimental conditions, such as an acidic solution or high sample flow rate (a parts per thousand yen200 mu L/min), more folded protein ions appear to be kept in droplets of diminishing size due to breakup by the high-speed nebulizer gas in ESSI compared with ESI. Theoretical analyses and numerical simulations were also performed to explain the observed phenomena. These systematic studies clarify the ionization mechanism of ESSI and provide valuable insight for optimizing ESSI and other popular pneumatically assisted electrospray ionization methods for future applications.
DOI 10.1007/s13361-011-0124-x
Additional Notes Received 10 December 2010, Revised 4 March 2011, Accepted 4 March 2011, Published online 22 April 2011
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03510
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NEBIS System Number 000541631
Source Database ID WOS-000291257400014
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  author = "Wang, Rui and Allmendinger, Pitt and Zhu, Liang and Groehn, Arto Juhani and Wegner, Karsten and Frankevich, Vladimir and Zenobi, Renato",
  title = "{T}he {R}ole of {N}ebulizer {G}as {F}low in {E}lectrosonic {S}pray {I}onization ({E}{S}{S}{I})",
  journal = "Journal of the American Society for Mass Spectrometry",
  year = 2011,
  volume = "22",
  number = "7",
  pages = "1234--1241",
  month = jul,

E-Citations record created: Mon, 04 Jul 2011, 13:03:23 CET