Electrosonic spray ionization

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Author(s) Schmid, Stefan, Jecklin, Matthias C., Zenobi, Renato
Publication Type Journal Items, Publication Status: Published
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Title Electrosonic spray ionization
Subtitle An ideal interface for high-flow liquid chromatography applications
Author(s) Schmid, Stefan
Jecklin, Matthias C.
Zenobi, Renato
Journal or Series Title Journal of chromatography. A
Volume Number 1218
Issue Number 23
Start Page 3704
End Page 3710
ISSN 0021-9673
Publisher Elsevier
Publication Place Amsterdam
Publication Date 2011-06
Keyword(s) High-flow liquid chromatography
Electrosonic spray ionization
Electrospray ionization
Mass spectrometry
LC-MS interface
Ambient ionization techniques
Abstract Electrosonic spray ionization (ESSI) has been studied as an interface between high-performance liquid chromatography (HPLC) and mass spectrometry (MS), using sample flow rates up to 3.0 ml min(-1). This ionization interface was compared with pneumatically assisted electrospray ionization (ESI) using mass spectrometry for detection. For experiments that did not involve direct comparison of different flow rates, the ESI experiments were performed using post column splitting to work at optimal conditions. ESSI allows the interfacing of conventional or high-resolution liquid chromatography (LC) methods to mass spectrometry without post column splitting. High sample flow rates could be handled without a significant loss of signal intensity using a nebulization gas flow rate of 5.5 L min(-1). Since ESI needs to be operated with lower sample flow rates, it is limited to micro/nano LC systems, or post column splitting must be used. In particular, nano LC systems have to be treated with great care and require constant maintenance. When using post-column splitting, the increased diffusion can become a problem especially when using systems with very small void volumes. In all experiments ESSI showed better signal intensities than a commercially available, pneumatically assisted ESI source. ESSI does not require heating of the nebulizer gas, which should help to preserve the original structure of thermally unstable molecules. Therefore. ESSI is presented as an alternative to the commercially available heated ESI sources of AB SCIEX,Thermo Fischer, Agilent and Waters. The observed LC-ESSI-MS ion chromatograms are shown to be very stable even when using flow rates higher than 1.0 ml min(-1), which could be very suitable for ultra high performance LC, where sample flow rates up to 2.0 mL min(-1) with backpressures up to 1200 bar are used. Also, a difference in the relative intensities of singly and doubly protonated peptide monomers and dimers was observed between the two ionization methods. The coefficients of determination for the calibration of instrument response for Val-Tyr-Val and Met-Enkephalin showed excellent linearity over a wide concentration range (0.1-100 mu M), while ESI results were only linear over a much smaller range (0.1-20 mu M). The observed behavior is thought to be caused by insufficient ionization efficiency of solutions above similar to 20 mu M by ESI, exemplifying the robustness of ESSI as an interface between LC and MS.
DOI 10.1016/j.chroma.2011.04.030
Additional Notes Received 22 September 2010, Revised 8 April 2011, Accepted 11 April 2011, Available online 16 April 2011
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03430
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NEBIS System Number 001820689
Source Database ID WOS-000291521200019
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  author = "Schmid, Stefan and Jecklin, Matthias C. and Zenobi, Renato",
  title = "{E}lectrosonic spray ionization: {A}n ideal interface for high-flow liquid chromatography applications",
  journal = "Journal of chromatography. A",
  year = 2011,
  volume = "1218",
  number = "23",
  pages = "3704--3710",
  month = jun,

E-Citations record created: Mon, 04 Jul 2011, 10:30:45 CET