Efficient electroporation of peptides into adherent cells

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Author(s) Schönenberger, Claudio, Schütz, Anette, Franco-Obregon, Alfredo, Zenobi-Wong, Marcy
Publication Type Journal Items, Publication Status: Published
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Title Efficient electroporation of peptides into adherent cells
Subtitle Investigation of the role of mechano-growth factor in chondrocyte culture
Author(s) Schönenberger, Claudio
Schütz, Anette
Franco-Obregon, Alfredo
Zenobi-Wong, Marcy
Journal or Series Title Biotechnology letters
Volume Number 33
Issue Number 5
Start Page 883
End Page 888
ISSN 0141-5492
1573-6776
Publisher Springer
Publication Place Dordrecht
Publication Date 2011-05
Keyword(s) Chondrocytes
In situ electroporation
Indium tin oxide
Mechano-growth factor
Peptide therapeutics
Abstract Peptide therapeutics are of increasing interest due to their biological specificity. We used a simple technique to study the efficacy of inducing peptides into adherent chondrocytes by transiently permeabilizing the membrane with electric pulses (in situ electroporation). Mechano-growth factor (MGF) was selected as a model peptide. FITC-labeled MGF was added to cultures of adherent primary chondrocytes grown on ITO coated glass slides. Cells were subjected to 3-9 pulses of 175-275 V and evaluated by flow cytometry. Under optimal conditions, an electroporation efficiency of close to 50% could be achieved. This technique can be used to study the functional domains of intracellular peptides, peptide inhibition of signal transduction and intracrine-mediated effects of peptides in adherent cells.
DOI 10.1007/s10529-010-0508-1
Additional Notes Received 31 August 2010, Accepted 20 December 2010, Published online 5 January 2011
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03741
Organisational Unit(s)
NEBIS System Number 000007236
Source Database ID WOS-000289897100003
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@article{Schnnbrgr2011,
  author = "Sch{\"{o}}nenberger, Claudio and Sch{\"{u}}tz, Anette and Franco-Obregon, Alfredo and Zenobi-Wong, Marcy",
  title = "{E}fficient electroporation of peptides into adherent cells: {I}nvestigation of the role of mechano-growth factor in chondrocyte culture",
  journal = "Biotechnology letters",
  year = 2011,
  volume = "33",
  number = "5",
  pages = "883--888",
  month = may,
}


E-Citations record created: Mon, 23 May 2011, 06:54:20 CET