Layer-by-Layer Films Made from Extracellular Matrix Macromolecules on Silicone Substrates

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Author(s) Mhanna, Rami F., Voeroes, Janos, Zenobi-Wong, Marcy
Publication Type Journal Items, Publication Status: Published
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Title Layer-by-Layer Films Made from Extracellular Matrix Macromolecules on Silicone Substrates
Author(s) Mhanna, Rami F.
Voeroes, Janos
Zenobi-Wong, Marcy
Journal or Series Title Biomacromolecules
Volume Number 12
Issue Number 3
Start Page 609
End Page 616
ISSN 1525-7797
Publisher American Chemical Society
Publication Place Washington
Publication Date 2011-03
Abstract The layer-by-layer (LbL) technique has been widely used to produce nanofilms for biomedical applications. Naturally occurring polymers such as ECM macromolecules are attractive candidates for LbL film preparation. In this study, we assessed the build-up of type I collagen (Col1)/chondroitin sulfate (CS) or Col1/Heparin (HN) on polydimethylsiloxane (PDMS) substrates. The build-up was assessed by quartz crystal microbalance with dissipation (QCM-D) and atomic force microscopy (AFM). Integrin-mediated cell adhesion was assessed by studying the cytoskeletal organization of mammalian primary cells (chondrocytes) seeded on different end layers and number of layers. Data generated from the QCM-D observations showed a consistent build-up of films with more adsorption in the case of Col1/HN. Col1/CS films were stable in media, whereas Col1/HN films were not. AFM analysis showed that the layers were fibrillar in structure for both systems and between 20 and 30 nm thick. The films promoted cell adhesion when compared with tissue culture plastic in serum-free media with cycloheximide. Crosslinking of the films resulted in constrained cell spreading and a ruffled morphology. Finally, betal integrin blocking antibodies prevented cell spreading, suggesting that cell adhesion and spreading were mediated mainly by interaction with the collagen fibrils. The ability to construct stable ECM-based films on PDMS has particular relevance in mechanobiology, microfluidics, and other biomedical applications.
DOI 10.1021/bm1012772
Additional Notes Published In Issue 14 March 2011, Article ASAP 14 February 2011, Received 25 October 2010, Revised 17 January 2011
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03741
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NEBIS System Number 003899084
Source Database ID WOS-000288183100012
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  author = "Mhanna, Rami F. and Voeroes, Janos and Zenobi-Wong, Marcy",
  title = "{L}ayer-by-{L}ayer {F}ilms {M}ade from {E}xtracellular {M}atrix {M}acromolecules on {S}ilicone {S}ubstrates",
  journal = "Biomacromolecules",
  year = 2011,
  volume = "12",
  number = "3",
  pages = "609--616",
  month = mar,

E-Citations record created: Mon, 04 Apr 2011, 09:05:14 CET