Determination of zinc to beta-peptide binding constants with electrospray ionization mass spectrometry

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Author(s) Wortmann, Arno, Rossi, Francesco, Lelais, Gerald, Zenobi, Renato
Publication Type Journal Items, Publication Status: Published
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Title Determination of zinc to beta-peptide binding constants with electrospray ionization mass spectrometry
Author(s) Wortmann, Arno
Rossi, Francesco
Lelais, Gerald
Zenobi, Renato
Journal or Series Title Journal of mass spectrometry
Volume Number 40
Issue Number 6
Start Page 777
End Page 784
ISSN 1076-5174
1076-5164
1096-9888
Publisher Wiley
Publication Place Chichester
Publication Date 2005-06
Keyword(s) Electrospray ionization mass spectrometry
Noncovalent complexes
Binding constant
Beta-peptides
Metal-peptide complexes
Abstract We present an improvement of the titration method for binding constant determination with electrospray ionization (ESI) mass spectrometry that is unaffected by differences in ESI response of measured species in solution. The method consists of a calibration and titration, both using an internal standard that allows relative quantitation. This avoids artifacts such as a decrease in overall signal intensity with increasing ligand concentrations, rendering this approach more reliable and meaningful than direct evaluation of ESI peak intensities. We demonstrate the de novo binding constant determination of novel zinc binding beta-peptides, which have been synthesized with the goal of creating secondary structures stabilized by metal complexation.
DOI 10.1002/jms.852
Additional Notes Received 15 November 2004, Accepted 14 February 2005
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03430
Organisational Unit(s)
NEBIS System Number 001410098
Source Database ID WOS-000230187600009
PP-17151
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@article{Wrtmnn2005,
  author = "Wortmann, Arno and Rossi, Francesco and Lelais, Gerald and Zenobi, Renato",
  title = "{D}etermination of zinc to beta-peptide binding constants with electrospray ionization mass spectrometry",
  journal = "Journal of mass spectrometry",
  year = 2005,
  volume = "40",
  number = "6",
  pages = "777--784",
  month = jun,
}


E-Citations record created: Fri, 01 Apr 2011, 07:51:51 CET