Clear evidence of fluorescence resonance energy transfer in gas-phase ions

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Author(s) Dashtiev, Maxim, Azov, Vladimir, Frankevich, Vladimir, Scharfenberg, Ludwig, Zenobi, Renato
Publication Type Journal Items, Publication Status: Published
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Title Clear evidence of fluorescence resonance energy transfer in gas-phase ions
Author(s) Dashtiev, Maxim
Azov, Vladimir
Frankevich, Vladimir
Scharfenberg, Ludwig
Zenobi, Renato
Journal or Series Title Journal of the American Society for Mass Spectrometry
Volume Number 16
Issue Number 9
Start Page 1481
End Page 1487
ISSN 1044-0305
Publisher Springer
Publication Place New York
Publication Date 2005-09
Abstract Fluorescence resonance energy transfer (FRET) is a distance-sensitive method that correlates changes in fluorescence intensity with conformational changes, for example, of biomolecules in the cellular environment. Applied to the gas phase in combination with Fourier transform ion cyclotron resonance mass spectrometry, it opens up possibilities to define structural/conformational properties of molecular ions, in the absence of solvent, and without the need for purification of the sample. For successfully observing FRET in the gas phase it is important to find suitable fluorophores. In this study several fluorescent dyes were examined, and the correlation between solution-phase and gas-phase fluorescence data were studied. For the first time, FRET in the gas phase is demonstrated unambiguously.
DOI 10.1016/j.jasms.2005.04.012
Additional Notes Published online 14 July 2005
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03430
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NEBIS System Number 000541631
Source Database ID WOS-000231646500009
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  author = "Dashtiev, Maxim and Azov, Vladimir and Frankevich, Vladimir and Scharfenberg, Ludwig and Zenobi, Renato",
  title = "{C}lear evidence of fluorescence resonance energy transfer in gas-phase ions",
  journal = "Journal of the American Society for Mass Spectrometry",
  year = 2005,
  volume = "16",
  number = "9",
  pages = "1481--1487",
  month = sep,

E-Citations record created: Wed, 23 Mar 2011, 10:03:54 CET