Multidimensional Analysis of Single Algal Cells by Integrating Microspectroscopy with Mass Spectrometry

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Author(s) Urban, Pawel L., Schmid, Thomas, Amantonico, Andrea, Zenobi, Renato
Publication Type Journal Items, Publication Status: Published
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Title Multidimensional Analysis of Single Algal Cells by Integrating Microspectroscopy with Mass Spectrometry
Author(s) Urban, Pawel L.
Schmid, Thomas
Amantonico, Andrea
Zenobi, Renato
Journal or Series Title Analytical chemistry
Volume Number 83
Issue Number 5
Start Page 1843
End Page 1849
ISSN 0003-2700
Publisher American Chemical Society
Publication Place Washington, DC
Publication Date 2011-03
Abstract We demonstrate a facile label-free approach for performing multidimensional chemical analysis on individual single-cell organisms by combining optical, fluorescence, and Raman microspectroscopy with matrix-free laser desorption/ionization mass spectrometry (MS). Single unicellular algae are seeded on a bare stainless steel plate and analyzed microspectroscopically. This provides information on the content and distribution of photoactive species, such as beta-carotene, as well as chlorophyll and other components of the photosynthetic apparatus. Exactly the same cells are then analyzed by mass spectrometry in the negative ion mode. Phospholipid species are readily ionized by laser desorption/ionization of intact cells, without the need for an auxiliary matrix. This not only facilitates sample preparation but also preserves high spatial resolution and high sensitivity. Using this method, we were able to study the content and arrangement of proplastids and photosystem components, as well as the amounts of various phospholipid species in individual algal cells. The methodology can be used in the fundamental biological studies on these unicellular organisms, which require information on the internal structure as well as the chemical composition of individual cells.
DOI 10.1021/ac102702m
Additional Notes Received 12 October 2010, Accepted 23 December 2010, Published online 7 February 2011
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03430
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NEBIS System Number 000005465
Source Database ID WOS-000287685800051
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  author = "Urban, Pawel L. and Schmid, Thomas and Amantonico, Andrea and Zenobi, Renato",
  title = "{M}ultidimensional {A}nalysis of {S}ingle {A}lgal {C}ells by {I}ntegrating {M}icrospectroscopy with {M}ass {S}pectrometry",
  journal = "Analytical chemistry",
  year = 2011,
  volume = "83",
  number = "5",
  pages = "1843--1849",
  month = mar,

E-Citations record created: Mon, 21 Mar 2011, 13:22:32 CET