Interfacing liquid chromatography with atmospheric pressure MALDI-MS

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Author(s) Daniel, Jürg M., Laiko, Victor V., Doroshenko, Vladimir M., Zenobi, Renato
Publication Type Journal Items, Publication Status: Published
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Title Interfacing liquid chromatography with atmospheric pressure MALDI-MS
Author(s) Daniel, Jürg M.
Laiko, Victor V.
Doroshenko, Vladimir M.
Zenobi, Renato
Journal or Series Title Analytical and bioanalytical chemistry
Volume Number 383
Issue Number 6
Start Page 895
End Page 902
ISSN 1618-2642
Publisher Springer
Publication Place Berlin
Publication Date 2005-11
Keyword(s) Matrix assisted laser desorption/ionization
IR ionization from solution
IR matrix assisted laser desorption/ionization
Liquid matrix
Atmospheric pressure matrix assisted laser desorption/ionization
Abstract Two different strategies for coupling liquid chromatography with atmospheric pressure matrix assisted laser desorption/ionization (AP MALDI) are presented. The first method is flow-injection liquid AP UV-MALDI. Compared with previous similar research, the detection limit was improved 10 times to 8.3 fmol using a solution of 50 nM peptide with 25 mM alpha-cyano-4-hydroxycinnamic acid. The applicability of this method to measure oligosaccharides, actinomycin antibiotics, antibiotics, phosphopeptides, and proteins is demonstrated. The upper mass limit achieved with the current instrumentation is 6,500 Da (doubly charged cytochrome c). The feasibility of a second strategy based on single-droplet IR AP MALDI is demonstrated here. Aqueous peptide solutions were successfully measured by this method.
DOI 10.1007/s00216-005-0098-2
Additional Notes Received 16 June 2005, Revised 18 August 2005, Accepted 31 August 2005, Published online 28 October 2005
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03430
Organisational Unit(s)
NEBIS System Number 004239219
Source Database ID WOS-000233875200001
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  author = "Daniel, J{\"{u}}rg M. and Laiko, Victor V. and Doroshenko, Vladimir M. and Zenobi, Renato",
  title = "{I}nterfacing liquid chromatography with atmospheric pressure {M}{A}{L}{D}{I}-{M}{S}",
  journal = "Analytical and bioanalytical chemistry",
  year = 2005,
  volume = "383",
  number = "6",
  pages = "895--902",
  month = nov,

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