Electrochemically switchable platform for the micro-patterning and release of heterotypic cell sheets

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Author(s) Guillaume-Gentil, Orane, Gabi, Michael, Zenobi-Wong, Marcy, Vörös, Janos
Publication Type Journal Items, Publication Status: Published
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Title Electrochemically switchable platform for the micro-patterning and release of heterotypic cell sheets
Author(s) Guillaume-Gentil, Orane
Gabi, Michael
Zenobi-Wong, Marcy
Vörös, Janos
Journal or Series Title Biomedical microdevices
Volume Number 13
Issue Number 1
Start Page 221
End Page 230
ISSN 1387-2176
1572-8781
Publisher Springer
Publication Place Dordrecht
Publication Date 2011-02
Keyword(s) Indium tin oxide electrode
SU-8 photoresist
Micro-patterning
Photolithography
Cell co-cultures
Heterotypic cell sheet engineering
Abstract This article describes a dynamic platform in which the biointerfacial properties of micro-patterned domains can be switched electrochemically through the spatio-temporally controlled dissolution and adsorption of polyelectrolyte coatings. Insulating SU-8 micro-patterns created on a transparent indium tin oxide electrode by photolithography allowed for the local control over the electrochemical dissolution of polyelectrolyte mono- and multilayers, with polyelectrolytes shielded from the electrochemical treatment by the underlying photoresist stencil. The platform allowed for the creation of micro-patterned cell co-cultures through the electrochemical removal of a non-fouling polyelectrolyte coating and the localized adsorption of a cell adhesive one after attachment of the first cell population. In addition, the use of weak adhesive polyelectrolyte coatings on the photoresist domains allowed for the detachment of a contiguous heterotypic cell sheet upon electrochemical trigger. Cells grown on the ITO domains peeled off upon electrochemical dissolution of the sacrificial polyelectrolyte substrate, whereas adjacent cell areas on the insulated weakly adhesive substrate easily detached through the contractile force generated by neighboring cells. This electrochemical strategy for the micro-patterning and detachment of heterotypic cell sheets combines simplicity, precision and versatility, and presents great prospects for the creation of cellular constructs which mimic the cellular complexity of native tissues.
DOI 10.1007/s10544-010-9487-1
Additional Notes Published online 6 November 2010
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03741
Organisational Unit(s)
NEBIS System Number 004387461
Source Database ID WOS-000286627000023
FORM-1295441629
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@article{Gllm2011,
  author = "Guillaume-Gentil, Orane and Gabi, Michael and Zenobi-Wong, Marcy and V{\"{o}}r{\"{o}}s, Janos",
  title = "{E}lectrochemically switchable platform for the micro-patterning and release of heterotypic cell sheets",
  journal = "Biomedical microdevices",
  year = 2011,
  volume = "13",
  number = "1",
  pages = "221--230",
  month = feb,
}


E-Citations record created: Mon, 28 Feb 2011, 08:13:52 CET