Analytical techniques for single-cell metabolomics

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Author(s) Amantonico, Andrea, Urban, Pawel L., Zenobi, Renato
Publication Type Journal Items, Publication Status: Published
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Title Analytical techniques for single-cell metabolomics
Subtitle State of the art and trends
Author(s) Amantonico, Andrea
Urban, Pawel L.
Zenobi, Renato
Journal or Series Title Analytical and bioanalytical chemistry
Volume Number 398
Issue Number 6
Start Page 2493
End Page 2504
ISSN 1618-2642
Publisher Springer
Publication Place Berlin
Publication Date 2010-12
Keyword(s) Cell individuality
Cell-to-cell variability
Single-cell analysis
Small-molecule analysis
Ultrasensitive analysis
Abstract Single-cell metabolomics is an emerging field that addresses fundamental biological questions and allows one to observe metabolic phenomena in heterogeneous populations of single cells. In this review, we assess the suitability of different detection techniques and present considerations on sample preparation for single-cell metabolomics. Although targeted analysis of single cells can readily be conducted using fluorescent probes and optical instruments (microscopes, fluorescence detectors), a comprehensive metabolomic approach requires a powerful label-free method, such as mass spectrometry (MS). Mass-spectrometric techniques applied to study small molecules in single cells include electrospray MS, matrix-assisted laser desorption/ionization MS, and secondary ion MS. Sample preparation is an important aspect to be taken into account during further development of methods for single-cell metabolomics.
DOI 10.1007/s00216-010-3850-1
Additional Notes Received 30 March 2010, Revised 9 May 2010, Accepted 13 May 2010, Published online 11 June 2010
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03430
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NEBIS System Number 004239219
Source Database ID WOS-000283588600014
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  author = "Amantonico, Andrea and Urban, Pawel L. and Zenobi, Renato",
  title = "{A}nalytical techniques for single-cell metabolomics: {S}tate of the art and trends",
  journal = "Analytical and bioanalytical chemistry",
  year = 2010,
  volume = "398",
  number = "6",
  pages = "2493--2504",
  month = dec,

E-Citations record created: Tue, 23 Nov 2010, 12:50:02 CET