Single-Cell MALDI-MS as an Analytical Tool for Studying Intrapopulation Metabolic Heterogeneity of Unicellular Organisms

Metadata Label Value
Author(s) Amantonico, Andrea, Urban, Pawel L., Fagerer, Stephan R., Balabin, Roman M., Zenobi, Renato
Publication Type Journal Items, Publication Status: Published
Full Text Search SFX for a Full-Text version of this document
Import to Mendeley Log in to provide feedback

Detailed Information

Metadata Field Content
Title Single-Cell MALDI-MS as an Analytical Tool for Studying Intrapopulation Metabolic Heterogeneity of Unicellular Organisms
Author(s) Amantonico, Andrea
Urban, Pawel L.
Fagerer, Stephan R.
Balabin, Roman M.
Zenobi, Renato
Journal or Series Title Analytical Chemistry
Volume Number 82
Issue Number 17
Start Page 7394
End Page 7400
ISSN 0003-2700
Publisher American Chemical Society
Publication Place Washington D.C.
Publication Date 2010-09-01
Abstract Heterogeneity is a characteristic feature of all populations of living organisms. Here we make an attempt to validate a single-cell mass spectrometric method for detection of changes in metabolite levels occurring in populations of unicellular organisms. Selected metabolites involved in central metabolism (ADP, ATP, GTP, and UDP-Glucose) could readily be detected in single cells of Closterium acerosum by means of negative-mode matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS). The analytical capabilities of this approach were characterized using standard compounds. The method was then used to study populations of individual cells with different levels of the chosen metabolites. With principal component analysis and support vector machine algorithms, it was possible to achieve a clear separation of individual C. acerosum cells in different metabolic states. This study demonstrates the suitability of mass spectrometric analysis of metabolites in single cells to measure cell-population heterogeneity.
DOI 10.1021/ac1015326
Additional Notes Received 9 June 2010, Accepted 30 July 2010
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03430
Organisational Unit(s)
NEBIS System Number 000005465
Source Database ID WOS-000281306000047
Description File Name MIME Type Size
No details could be found
There are no links available for this record.
This record has not been viewed during this period

  author = "Amantonico, Andrea and Urban, Pawel L. and Fagerer, Stephan R. and Balabin, Roman M. and Zenobi, Renato",
  title = "{S}ingle-{C}ell {M}{A}{L}{D}{I}-{M}{S} as an {A}nalytical {T}ool for {S}tudying {I}ntrapopulation {M}etabolic {H}eterogeneity of {U}nicellular {O}rganisms",
  journal = "Analytical Chemistry",
  year = 2010,
  volume = "82",
  number = "17",
  pages = "7394--7400",
  month = sep,

E-Citations record created: Mon, 13 Sep 2010, 07:47:53 CET