Negative mode nanostructure-initiator mass spectrometry for detection of phosphorylated metabolites

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Author(s) Amantonico, Andrea, Flamigni, Luca, Glaus, Reto, Zenobi, Renato
Publication Type Journal Items, Publication Status: Published
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Title Negative mode nanostructure-initiator mass spectrometry for detection of phosphorylated metabolites
Author(s) Amantonico, Andrea
Flamigni, Luca
Glaus, Reto
Zenobi, Renato
Journal or Series Title Metabolomics
Volume Number 5
Issue Number 3
Start Page 346
End Page 353
ISSN 1573-3882
1573-3890
Publisher Springer
Publication Place Berlin
Publication Date 2009
Keyword(s) laser desorption/ionization mass spectometry
phosphorylated metabolites
nucleotide detection
nanostructure initiator mass spectrometry
porous silicon
Abstract The chemical complexity of the metabolome requires the development of new detection methods to enlarge the range of compounds detectable in a biological sample. Recently, a novel matrix-free laser desorption/ionization method called nanostructure-initiator mass spectrometry (NIMS) [Northen et al., Nature 449(7165):1033–1036, 2007] was reported. Here we investigate NIMS in negative ion mode for the detection of endogenous metabolites, namely small phosphorylated molecules. 3-Aminopropyldimethylethoxysilane was found to be suitable as initiator for the analytes studied and a limit of detection in the tens of femtomoles was reached. The detection of different endogenous cell metabolites in a yeast cell extract is demonstrated.
DOI 10.1007/s11306-009-0163-5
Additional Notes Received 15 December 2008, Accepted 2 April 2009, Published online 27 May 2009
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03430
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NEBIS System Number 005032934
Source Database ID PP-49378
WOS-000269312400008
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@article{mntnc2009,
  author = "Amantonico, Andrea and Flamigni, Luca and Glaus, Reto and Zenobi, Renato",
  title = "{N}egative mode nanostructure-initiator mass spectrometry for detection of phosphorylated metabolites",
  journal = "Metabolomics",
  year = 2009,
  volume = "5",
  number = "3",
  pages = "346--353",
}


E-Citations record created: Fri, 02 Apr 2010, 03:46:13 CET