Interfacing Microfluidics and Laser Desorption/Ionization Mass Spectrometry by Continuous Deposition for Application in Single Cell Analysis

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Author(s) Amantonico, Andrea, Urban, Pawel L., Oh, Joo Yeon, Zenobi, Renato
Publication Type Journal Items, Publication Status: Published
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Title Interfacing Microfluidics and Laser Desorption/Ionization Mass Spectrometry by Continuous Deposition for Application in Single Cell Analysis
Author(s) Amantonico, Andrea
Urban, Pawel L.
Oh, Joo Yeon
Zenobi, Renato
Journal or Series Title Chimia
Volume Number 63
Issue Number 4
Start Page 185
End Page 188
ISSN 0009-4293
Publisher Swiss Chemical Society
Publication Place Bern
Publication Date 2009
Keyword(s) hyphenated techniques
mass spectrometry
microscale sample preparation
single cell analysis
writing on MALDI plates
Abstract We present a simple method for continuous deposition of effluent originating from a microfluidic device on a flat metal surface for subsequent analysis by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). The sample is delivered using a microscale fused silica capillary and passed onto the surface of a stainless steel plate coated with a layer of a standard matrix. The key parameters optimized in order to obtain high quality and reproducible sample traces are: i) sampleflow rate, ii) speed of the XY-stage movement, and iii) distance of the capillary tip from the plate. Tapering the capillary end as well as surface functionalization to induce hydrophobicity were shown to further enhance the deposition process. The described continuous deposition method is compared with a previously published mass spectrometric method utilizing a piezoelectric microdispenser for microspotting onto the MALDI plates which enabled detection of primary metabolites at the singlecell level. Research is underway to adapt the continuous deposition as an interface for single cell metabolite detection and enhancement of quantitative abilities of the MALDI methodology. We envisage that the presented continuous deposition method may also be suitable for sensitive detection of analytes using other surface analysis tools.
DOI 10.2533/chimia.2009.185
Additional Notes Received February 2 2009
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03430
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NEBIS System Number 000000830
Source Database ID PP-49096
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  author = "Amantonico, Andrea and Urban, Pawel L. and Oh, Joo Yeon and Zenobi, Renato",
  title = "{I}nterfacing {M}icrofluidics and {L}aser {D}esorption/{I}onization {M}ass {S}pectrometry by {C}ontinuous {D}eposition for {A}pplication in {S}ingle {C}ell {A}nalysis",
  journal = "Chimia",
  year = 2009,
  volume = "63",
  number = "4",
  pages = "185--188",

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