Towards chemical analysis of nanostructures in biofilms I

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Author(s) Schmid, Thomas, Burkhard, Johannes, Yeo, Boon-Siang, Zhang, Weihua, Zenobi, Renato
Publication Type Journal Items, Publication Status: Published
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Title Towards chemical analysis of nanostructures in biofilms I
Subtitle Imaging of biological nanostructures
Author(s) Schmid, Thomas
Burkhard, Johannes
Yeo, Boon-Siang
Zhang, Weihua
Zenobi, Renato
Journal or Series Title Analytical and bioanalytical chemistry
Volume Number 391
Issue Number 5
Start Page 1899
End Page 1905
ISSN 1618-2642
1618-2650
Publisher Springer
Publication Place Berlin
Publication Date 2008
Keyword(s) biofilm
extracellular polymeric substances (EPS)
alginate
confocal laser-scanning microscopy (CLSM)
atomic force microscopy (AFM)
Abstract Due to their direct influence on the stability of bacterial biofilms, a better insight into the nanoscopic spatial arrangement of the different extracellular polymeric substances (EPS), e.g., polysaccharides and proteins, is important for the improvement of biocides and for process optimization in wastewater treatment and biofiltration. Here, the first application of a combination of confocal laser-scanning microscopy (CLSM) and atomic force microscopy (AFM) to the investigation of river-water biofilms and related biopolymers is presented. AFM images collected at selected areas of CLS micrographs dramatically demonstrate the heterogeneity of biofilms at the nanometer scale and the need for a chemical imaging method with nanoscale resolution. The nanostructures (e.g., pili, flagella, hydrocolloids, and EPS) found in the extracellular matrix are classified according to shape and size, which is typically 50-150 nm in width and 1-10 nm in thickness, and sets the demands regarding spatial resolution of a potential chemical imaging method. Additionally, thin layers of the polysaccharide alginate were investigated. We demonstrate that calcium alginate is a good model for the EPS architecture at the nanometer scale, because of its similar network-like structure.
DOI 10.1007/s00216-008-2100-2
Additional Notes Received 23 December 2007, Revised 20 March 2008, Accepted 25 March 2008, Published online 22 April 2008
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03430
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NEBIS System Number 004239219
Source Database ID PP-41467
WOS-000256908200043
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@article{Schmd2008,
  author = "Schmid, Thomas and Burkhard, Johannes and Yeo, Boon-Siang and Zhang, Weihua and Zenobi, Renato",
  title = "{T}owards chemical analysis of nanostructures in biofilms {I}: {I}maging of biological nanostructures",
  journal = "Analytical and bioanalytical chemistry",
  year = 2008,
  volume = "391",
  number = "5",
  pages = "1899--1905",
}


E-Citations record created: Thu, 01 Apr 2010, 23:01:57 CET