# Neutral desorption sampling of biological surfaces for rapid chemical characterization by extractive electrospray ionization mass spectrometry

Author(s) Chen, Huanwen, Zenobi, Renato
Publication Type Journal Items, Publication Status: Published
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Title Neutral desorption sampling of biological surfaces for rapid chemical characterization by extractive electrospray ionization mass spectrometry
Author(s) Chen, Huanwen
Zenobi, Renato
Journal or Series Title Nature protocols
Volume Number 3
Start Page 1467
End Page 1475
ISSN 1750-2799
1745-2481
Publisher Nature Publ. Group
Publication Place Basingstoke
Publication Date 2008
Abstract Extractive electrospray ionization mass spectrometry (EESI-MS) allows the real-time, direct analysis of complex gaseous and liquid samples without any sample pretreatment under ambient conditions. By using a neutral desorption (ND) sampling gas beam to gently impact a surface, the analyte present on a surface can be efficiently sampled and then transported to the EESI source for soft ionization without any previous work-up. By separating the sampling process and the ionization process in both space and time, ion suppression effects are significantly decreased in ND-EESI-MS. Therefore, virtually any surface can be gently sampled in real time, allowing in vivo analysis of living objects while maintaining the native conditions of the sample. This greatly simplifies the procedure for the characterization of complex surfaces (e.g., plants, food, skin) and prevents potential chemical contamination, providing a unique platform for wide applications in multiple disciplines such as chemistry, biology and the life sciences. Here we present a complete description of a protocol for ND-EESI-MS for in vivo analysis of living objects, including equipment setup, reagent preparation, data acquisition and analysis steps. The data collection can be completed within a few minutes even though the time required for the entire analytical process, which largely depends on the preparation of samples and materials, takes slightly longer.
DOI 10.1038/nprot.2008.109
Additional Notes Published online 28 August 2008
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03430
Organisational Unit(s)
NEBIS System Number 005267183
Source Database ID PP-41453
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@article{Chn2008,
author = "Chen, Huanwen and Zenobi, Renato",
title = "{N}eutral desorption sampling of biological surfaces for rapid chemical characterization by extractive electrospray ionization mass spectrometry",
journal = "Nature protocols",
year = 2008,
volume = "3",
pages = "1467--1475",
}


E-Citations record created: Thu, 01 Apr 2010, 23:01:26 CET