Effects of N-glycosylation of the human cation channel TRPA1 on agonist-sensitivity

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Author(s) Egan, Timothy J., Acuña, Mario A., Zenobi-Wong, Marcy, Zeilhofer, Hanns Ulrich, Urech, David
Publication Type Journal Items, Publication Status: Published
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Title Effects of N-glycosylation of the human cation channel TRPA1 on agonist-sensitivity
Author(s) Egan, Timothy J.
Acuña, Mario A.
Zenobi-Wong, Marcy
Zeilhofer, Hanns Ulrich
Urech, David
Description 12 p.
Journal or Series Title Bioscience Reports
Volume Number 36
Issue Number 5
Start Page e00390
ISSN 0144-8463
1573-4935
Publisher Portland Press
Publication Place London
Publication Date 2016
Keyword(s) Glycoproteins
Glycosylation
Ion channels
Transient receptor potential channels
Abstract Determining the functional significance of post-translational modifications advances our understanding of many broadly-expressed proteins, and particularly ion channels. The enzymes that catalyse these modifications are often expressed in a cell-type specific manner, resulting in considerable structural diversity among post-translationally modified proteins that are expressed across a variety of cell types. TRP channels exhibit notably variable behaviour between cell types in vitro and in vivo, and they are frequently modified with N-glycans that contribute to protein function. TRPA1 possesses two putative N-linked glycosylation sites at Asn747 and Asn753 that have not yet been studied in detail. In the present study, we show that both of these sites can be modified with an N-glycan and that the glycan at position Asn747 modulates agonist-sensitivity of TRPA1 in vitro. Additionally, we found that N-glycosylation also modulates cooperative effects of temperature and the agonist cinnamaldehyde (CA) on TRPA1 channel activation. Collectively, these findings suggest a dynamic role played by the N-glycosylation of human TRPA1. They also provide further evidence of the versatility of N-glycans and will assist in efforts to fully understand the complex regulation of TRPA1 activity.
DOI 10.1042/bsr20160149
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03742
03949
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NEBIS System Number 000011835
Source Database ID WOS-000388364900014
CSV-pub:185985
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@article{gn2016,
  author = "Egan, Timothy J. and Acu{\~{n}}a, Mario A. and Zenobi-Wong, Marcy and Zeilhofer, Hanns Ulrich and Urech, David",
  title = "{E}ffects of {N}-glycosylation of the human cation channel {T}{R}{P}{A}1 on agonist-sensitivity",
  journal = "Bioscience Reports",
  year = 2016,
  volume = "36",
  number = "5",
  pages = "e00390--",
}


E-Citations record created: Mon, 19 Dec 2016, 15:19:56 CET