Influence of Ammonium Acetate Concentration on Receptor Ligand Binding Affinities Measured by Native Nano ESI-MS: A Systematic Study

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Author(s) Gavriilidou, Agni F. M., Gulbakan, Basri, Zenobi, Renato
Publication Type Journal Items, Publication Status: Published
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Title Influence of Ammonium Acetate Concentration on Receptor Ligand Binding Affinities Measured by Native Nano ESI-MS: A Systematic Study
Author(s) Gavriilidou, Agni F. M.
Gulbakan, Basri
Zenobi, Renato
Journal or Series Title Analytical chemistry
Volume Number 87
Issue Number 20
Start Page 10378
End Page 10384
ISSN 0003-2700
1520-6882
Publisher American Chemical Society
Publication Place Washington DC, USA
Publication Date 2015
Abstract Native electrospray ionization (ESI) mass spectrometry (MS) is a powerful technique for analyzing biomolecules in their native state. However, ESI-MS is incompatible with nonvolatile solution additives. Therefore, biomolecules have to be electrosprayed from a solution that differs from their purification or storage buffer, often aqueous ammonium acetate (AmAc). In this study, the effect of the ionic strength on the dissociation constants of six different noncovalent complexes, that cover interactions present in many biological systems, was investigated. Complexes were electrosprayed from 10 mM, SO mM, 100 mM, 300 mM, and 500 mM aqueous AmAc. For all systems, it was shown that the binding affinity is significantly influenced by the ionic strength of the solution. The determined dissociation constant (K-d) was affected more than 50% when increasing the AmAc concentration. The results are interpreted in terms of altered ionic interactions induced by the solution. This work emphasizes the modulating effect of the ions on noncovalent interactions and the importance of carefully choosing the AmAc concentration for quantifying the receptor ligand binding strengths.
DOI 10.1021/acs.analchem.5b02478
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03430
Organisational Unit(s)
NEBIS System Number 000005465
Source Database ID WOS-000363348300031
SCOPUS-84945405706
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@article{Gvrld2015,
  author = "Gavriilidou, Agni F. M. and Gulbakan, Basri and Zenobi, Renato",
  title = "{I}nfluence of {A}mmonium {A}cetate {C}oncentration on {R}eceptor {L}igand {B}inding {A}ffinities {M}easured by {N}ative {N}ano {E}{S}{I}-{M}{S}: {A} {S}ystematic {S}tudy",
  journal = "Analytical chemistry",
  year = 2015,
  volume = "87",
  number = "20",
  pages = "10378--10384",
}


E-Citations record created: Mon, 16 Nov 2015, 13:23:38 CET