Differentiation of oral bacteria in in vitro cultures and human saliva by secondary electrospray ionization: Mass spectrometry

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Author(s) Bregy, Lukas, Müggler, Annick R., Martinez-Lozano Sinues, Pablo, García-Gómez, Diego, Suter, Yannick, Belibasakis, Georgios N., Kohler, Malcolm, Schmidlin, Patrick R., Zenobi, Renato
Publication Type Journal Items, Publication Status: Published
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Title Differentiation of oral bacteria in in vitro cultures and human saliva by secondary electrospray ionization: Mass spectrometry
Author(s) Bregy, Lukas
Müggler, Annick R.
Martinez-Lozano Sinues, Pablo
García-Gómez, Diego
Suter, Yannick
Belibasakis, Georgios N.
Kohler, Malcolm
Schmidlin, Patrick R.
Zenobi, Renato
Description 10 p.
Journal or Series Title Scientific Reports
Volume Number 5
Start Page 15163
ISSN 2045-2322
Publisher Nature Publishing Group
Publication Place London
Publication Date 2015
Abstract The detection of bacterial-specific volatile metabolites may be a valuable tool to predict infection. Here we applied a real-time mass spectrometric technique to investigate differences in volatile metabolic profiles of oral bacteria that cause periodontitis. We coupled a secondary electrospray ionization (SESI) source to a commercial high-resolution mass spectrometer to interrogate the headspace from bacterial cultures and human saliva. We identified 120 potential markers characteristic for periodontal pathogens Aggregatibacter actinomycetemcomitans (n = 13), Porphyromonas gingivalis (n = 70), Tanerella forsythia (n = 30) and Treponema denticola (n = 7) in in vitro cultures. In a second proof-of-principle phase, we found 18 (P. gingivalis, T. forsythia and T. denticola) of the 120 in vitro compounds in the saliva from a periodontitis patient with confirmed infection with P. gingivalis, T. forsythia and T. denticola with enhanced ion intensity compared to two healthy controls. In conclusion, this method has the ability to identify individual metabolites of microbial pathogens in a complex medium such as saliva.
DOI 10.1038/srep15163
Additional Notes Accepted 17 September 2015, Published online 19 October 2015
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03430
Organisational Unit(s)
NEBIS System Number 006751867
Source Database ID SCOPUS-84945178342
WOS-000362979900001
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@article{Brgy2015,
  author = "Bregy, Lukas and M{\"{u}}ggler, Annick R. and Martinez-Lozano Sinues, Pablo and Garc{\'{i}}a-G{\'{o}}mez, Diego and Suter, Yannick and Belibasakis, Georgios N. and Kohler, Malcolm and Schmidlin, Patrick R. and Zenobi, Renato",
  title = "{D}ifferentiation of oral bacteria in in vitro cultures and human saliva by secondary electrospray ionization: {M}ass spectrometry",
  journal = "Scientific Reports",
  year = 2015,
  volume = "5",
  pages = "15163--",
}


E-Citations record created: Mon, 09 Nov 2015, 13:26:59 CET