Femtomolar oligonucleotide detection by a one-step gold nanoparticle-based assay

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Author(s) Rajendran, Prayanka, Kaufmann, Silvan, Vörös, János, Zenobi-Wong, Marcy, Demkó, László
Publication Type Journal Items, Publication Status: Published
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Title Femtomolar oligonucleotide detection by a one-step gold nanoparticle-based assay
Author(s) Rajendran, Prayanka
Kaufmann, Silvan
Vörös, János
Zenobi-Wong, Marcy
Demkó, László
Journal or Series Title Colloids and Surfaces B: Biointerfaces
Volume Number 135
Start Page 193
End Page 200
ISSN 0927-7765
1873-4367
Publisher Elsevier
Publication Place Amsterdam
Publication Date 2015-11
Keyword(s) DNA sensing
Femtomolar detection
Oligonucleotide
Gold nanoparticle
Local surface plasmon resonance
Biosensor
Abstract A sequence-specific oligonucleotide detection method based on the tail-to-tail aggregation of functionalized gold nanoparticles in the presence of target analytes is presented together with its optimization and capabilities for detection of single nucleotide polymorphisms (SNPs). In this single-step method, capture probes are freely accessible for hybridization, resulting in an improved assay performance compared to substrate-based assays. The analytes bring the nanoparticles close to each other via hybridization, causing a red shift of the nanoparticle plasmon peak detected by a spectrophotometer or CCD camera coupled to a darkfield imaging system. Optimal conditions for the assay were found to be (i) use of capture probes complementary to the target without any gap, (ii) maximum possible probe density on the gold nanoparticles, and (iii) 1 M ionic strength buffer. The optimized assay has a 1 fM limit of detection and fM to 10 pM dynamic range, with detection of perfect match sequences being three orders of magnitude more sensitive than targets with single nucleotide mismatches.
DOI 10.1016/j.colsurfb.2015.07.035
Additional Notes Published online 19 July 2015
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03949
03741
Organisational Unit(s)
NEBIS System Number 000896277
Source Database ID FORM-1442997384
WOS-000367410200023
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@article{Rjndrn2015,
  author = "Rajendran, Prayanka and Kaufmann, Silvan and V{\"{o}}r{\"{o}}s, János and Zenobi-Wong, Marcy and Demk{\'{o}}, L{\'{a}}szl{\'{o}}",
  title = "{F}emtomolar oligonucleotide detection by a one-step gold nanoparticle-based assay",
  journal = "Colloids and Surfaces B: Biointerfaces",
  year = 2015,
  volume = "135",
  pages = "193--200",
  month = nov,
}


E-Citations record created: Wed, 23 Sep 2015, 08:36:30 CET