Resolution pattern for mass spectrometry imaging

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Author(s) Fagerer, Stephan R., Römpp, Andreas, Jefimovs, Konstantins, Brönnimann, Rolf, Hayenga, Gerd, Steinhoff, Robert F., Krismer, Jasmin, Pabst, Martin, Ibanez, Alfredo J., Zenobi, Renato
Publication Type Journal Items, Publication Status: Published
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Title Resolution pattern for mass spectrometry imaging
Author(s) Fagerer, Stephan R.
Römpp, Andreas
Jefimovs, Konstantins
Brönnimann, Rolf
Hayenga, Gerd
Steinhoff, Robert F.
Krismer, Jasmin
Pabst, Martin
Ibanez, Alfredo J.
Zenobi, Renato
Journal or Series Title Rapid Communications in Mass Spectrometry (RCM)
Volume Number 29
Issue Number 11
Start Page 1019
End Page 1024
ISSN 0951-4198
Publisher Wiley
Publication Place London
Publication Date 2015-06
Up to now, there is no 'gold standard' for determining the resolution of a mass spectrometry imaging (MSI) setup (comprising the instrument, the sample preparation, the sample and the instrument settings). A standard sample in combination with a standard protocol to define the MSI resolution would be desirable in order to compare the setups of different laboratories, and as a regular quality control/performance check.

Microstructured resolution patterns were fabricated that can be used to determine the spatial resolution in MSI experiments, down to the range of a few mu m. Two different strategies were employed, one where the resolution pattern is laser machined into a thin metal foil, which can be placed over a sample to be imaged, and a second one where hydrophilic grooves are machined into an omniphobic coating covering the surface of an indium tin oxide covered glass slide. When dragging a sample solution over the slide's surface, the sample is automatically retained in the hydrophilic grooves, but repelled by the omniphobic coating.

The technology was tested on a commercial matrix-assisted laser desorption/ionization (MALDI) imaging instrument, and a spatial resolution in the vicinity of 50 mu m was determined. The finest features of the microstructured resolution patterns are compatible with the best spatial resolution of MALDI imaging systems available to date.

The use of metal resolution grids or glass slides with hydrophilic/hydrophobic structures is suitable for the convenient determination of the resolution limit of the MALDI imaging instrument as determined by its hardware. These structures are straightforward both to produce and to use.
DOI 10.1002/rcm.7191
Additional Notes Received 30 October 2014, Revised 5 February 2015, Accepted 7 March 2015, Published online 20 April 2015
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03430
Organisational Unit(s)
NEBIS System Number 000038989
Source Database ID WOS-000354566900004
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  author = "Fagerer, Stephan R. and R{\"{o}}mpp, Andreas and Jefimovs, Konstantins and Br{\"{o}}nnimann, Rolf and Hayenga, Gerd and Steinhoff, Robert F. and Krismer, Jasmin and Pabst, Martin and Ibanez, Alfredo J. and Zenobi, Renato",
  title = "{R}esolution pattern for mass spectrometry imaging",
  journal = "Rapid Communications in Mass Spectrometry (RCM)",
  year = 2015,
  volume = "29",
  number = "11",
  pages = "1019--1024",
  month = jun,

E-Citations record created: Mon, 08 Jun 2015, 07:41:35 CET