Applying Mass Spectrometry to Study Non-covalent Biomolecule Complexes

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Author(s) Chen, Fan, Gülbakan, Basri, Weidmann, Simon, Fagerer, Stephan R., Ibáñez, Alfredo J., Zenobi, Renato
Publication Type Journal Items, Publication Status: Published
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Title Applying Mass Spectrometry to Study Non-covalent Biomolecule Complexes
Author(s) Chen, Fan
Gülbakan, Basri
Weidmann, Simon
Fagerer, Stephan R.
Ibáñez, Alfredo J.
Zenobi, Renato
Journal or Series Title Mass Spectrometry Reviews
Volume Number 35
Issue Number 1
Start Page 48
End Page 70
ISSN 0277-7037
Publisher Wiley
Publication Place New York
Publication Date 2015-01
Keyword(s) Non-covalent interactions
First shot phenomenon
Chemical cross-linking
Abstract Non-covalent interactions are essential for the structural organization of biomacromolecules and play an important role in molecular recognition processes, such as the interactions between proteins, glycans, lipids, DNA, and RNA. Mass spectrometry (MS) is a powerful tool for studying of non-covalent interactions, due to the low sample consumption, high sensitivity, and label-free nature. Nowadays, native-ESI MS is heavily used in studies of non-covalent interactions and to understand the architecture of biomolecular complexes. However, MALDI-MS is also becoming increasingly useful. It is challenging to detect the intact complex without fragmentation when analyzing non-covalent interactions with MALDI-MS. There are two methodological approaches to do so. In the first approach, different experimental and instrumental parameters are fine-tuned in order to find conditions under which the complex is stable, such as applying non-acidic matrices and collecting first-shot spectra. In the second approach, the interacting species are “artificially” stabilized by chemical crosslinking. Both approaches are capable of studying non-covalently bound biomolecules even in quite challenging systems, such as membrane protein complexes. Herein, we review and compare native-ESI and MALDI MS for the study of non-covalent interactions.
DOI 10.1002/mas.21462
Additional Notes Received 6 June 2014, Accepted 9 December 2014, Published online 6 May 2015
Document Type Review Article
Publication Status Published
Language English
NEBIS System Number 000015310
Source Database ID SCOPUS-84955212522
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  author = "Chen, Fan and G{\"{u}}lbakan, Basri and Weidmann, Simon and Fagerer, Stephan R. and Ib{\'{a}}{\~{n}}ez, Alfredo J. and Zenobi, Renato",
  title = "{A}pplying {M}ass {S}pectrometry to {S}tudy {N}on-covalent {B}iomolecule {C}omplexes",
  journal = "Mass Spectrometry Reviews",
  year = 2015,
  volume = "35",
  number = "1",
  pages = "48--70",
  month = jan,

E-Citations record created: Wed, 27 May 2015, 12:09:21 CET