Plasma ionization source for atmospheric pressure mass spectrometry imaging using near-field optical laser ablation

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Author(s) Nudnova, Maryia M., Sigg, Jérôme, Wallimann, Pascal, Zenobi, Renato
Publication Type Journal Items, Publication Status: Published
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Title Plasma ionization source for atmospheric pressure mass spectrometry imaging using near-field optical laser ablation
Author(s) Nudnova, Maryia M.
Sigg, Jérôme
Wallimann, Pascal
Zenobi, Renato
Journal or Series Title Analytical Chemistry
Volume Number 87
Issue Number 2
Start Page 1323
End Page 1329
ISSN 0003-2700
1520-6882
Publisher American Chemical Society
Publication Place Washington, DC
Publication Date 2015-01
Abstract Mass spectrometry imaging (MSI) at ambient pressures with submicrometer resolution is challenging, due to the very low amount of material available for mass spectrometric analysis. In this work, we present the development and characterization of a method for MSI based on pulsed laser ablation via a scanning near-field optical microscopy (SNOM) aperture tip. SNOM allows laser ablation of material from surfaces with submicrometer spatial resolution, which can be ionized for further chemical analysis with MS. Efficient ionization is realized here with a custom-built capillary plasma ionization source. We show the applicability of this setup for mass spectrometric analysis of three common MALDI matrices, α-4-hydroxycyanocinnamic acid, 3-aminobenzoic acid, and 2,5-dihydroxybenzoic acid. Although the ultimate goal has been to optimize sensitivity for detecting material ablated from submicrometer diameter craters, the effective lateral resolution is currently limited by the sensitivity of the MS detection system. In our case, the sensitivity of the MS was about 1 fmol, which allowed us to achieve a spatial resolution of 2 μm. We also characterize the analytical figures of merit of our method. In particular, we demonstrate good reproducibility, a repetition rate in the range of only a few seconds, and we determined the amount of substance required to achieve optimal resolution and sensitivity. Moreover, the sample topography is available from SNOM scans, a parameter that is missing in common MSI methods.
DOI 10.1021/ac504039w
Additional Notes Received 29 October 2014, Accepted 17 December 2014, Published online 17 December 2014
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03430
Organisational Unit(s)
NEBIS System Number 000005465
Source Database ID SCOPUS-84922311549
WOS-000348332300067
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@article{Ndnv2015,
  author = "Nudnova, Maryia M. and Sigg, J{\'{e}}r{\^{o}}me and Wallimann, Pascal and Zenobi, Renato",
  title = "{P}lasma ionization source for atmospheric pressure mass spectrometry imaging using near-field optical laser ablation",
  journal = "Analytical Chemistry",
  year = 2015,
  volume = "87",
  number = "2",
  pages = "1323--1329",
  month = jan,
}


E-Citations record created: Tue, 17 Feb 2015, 08:57:55 CET