Circadian variation of the human metabolome captured by real-time breath analysis

Metadata Label Value
Author(s) Martinez-Lozano Sinues, Pablo, Tarokh, Leila, Li, Xue, Kohler, Malcolm, Brown, Steven A., Zenobi, Renato, Dallmann, Robert
Publication Type Journal Items, Publication Status: Published
Full Text Search SFX for a Full-Text version of this document
Import to Mendeley

Detailed Information

Metadata Field Content
Title Circadian variation of the human metabolome captured by real-time breath analysis
Author(s) Martinez-Lozano Sinues, Pablo
Tarokh, Leila
Li, Xue
Kohler, Malcolm
Brown, Steven A.
Zenobi, Renato
Dallmann, Robert
Description 16 p.
Journal or Series Title PLoS ONE
Volume Number 9
Issue Number 12
Start Page e114422
ISSN 1932-6203
Publisher Public Library of Science
Publication Place San Francisco, CA
Publication Date 2014-12
Abstract Circadian clocks play a significant role in the correct timing of physiological metabolism, and clock disruption might lead to pathological changes of metabolism. One interesting method to assess the current state of metabolism is metabolomics. Metabolomics tries to capture the entirety of small molecules, i.e. the building blocks of metabolism, in a given matrix, such as blood, saliva or urine. Using mass spectrometric approaches we and others have shown that a significant portion of the human metabolome in saliva and blood exhibits circadian modulation; independent of food intake or sleep/wake rhythms. Recent advances in mass spectrometry techniques have introduced completely non-invasive breathprinting; a method to instantaneously assess small metabolites in human breath. In this proof-of-principle study, we extend these findings about the impact of circadian clocks on metabolomics to exhaled breath. As previously established, our method allows for real-time analysis of a rich matrix during frequent non-invasive sampling. We sampled the breath of three healthy, non-smoking human volunteers in hourly intervals for 24 hours during total sleep deprivation, and found 111 features in the breath of all individuals, 36–49% of which showed significant circadian variation in at least one individual. Our data suggest that real-time mass spectrometric "breathprinting" has high potential to become a useful tool to understand circadian metabolism, and develop new biomarkers to easily and in real-time assess circadian clock phase and function in experimental and clinical settings.
DOI 10.1371/journal.pone.0114422
Additional Notes Received 7 August 2014, Accepted 10 November 2014, Published 29 December 2014
Document Type Article
Publication Status Published
Language English
NEBIS System Number 006206116
Source Database ID SCOPUS-84919935807
Description File Name MIME Type Size
No details could be found
There are no links available for this record.
This record has not been viewed during this period

  author = "Martinez-Lozano Sinues, Pablo and Tarokh, Leila and Li, Xue and Kohler, Malcolm and Brown, Steven A. and Zenobi, Renato and Dallmann, Robert",
  title = "{C}ircadian variation of the human metabolome captured by real-time breath analysis",
  journal = "PLoS ONE",
  year = 2014,
  volume = "9",
  number = "12",
  pages = "e114422--",
  month = dec,

E-Citations record created: Tue, 20 Jan 2015, 09:53:36 CET