Catalytic diesel particulate filters reduce the in vitro estrogenic activity of diesel exhaust

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Author(s) Wenger, Daniela, Gerecke, Andreas C., Heeb, Norbert V., Naegeli, Hanspeter, Zenobi, Renato
Publication Type Journal Items, Publication Status: Published
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Title Catalytic diesel particulate filters reduce the in vitro estrogenic activity of diesel exhaust
Author(s) Wenger, Daniela
Gerecke, Andreas C.
Heeb, Norbert V.
Naegeli, Hanspeter
Zenobi, Renato
Journal or Series Title Analytical and bioanalytical chemistry
Volume Number 390
Issue Number 8
Start Page 2021
End Page 2029
ISSN 1618-2642
Publisher Springer
Publication Place Berlin
Publication Date 2008
Keyword(s) Diesel exhaust
Diesel particles
Diesel particulate filter
In vitro reporter gene assay
Estrogen receptor
Estrogenic activity
Abstract An in vitro reporter gene assay based on human breast cancer T47D cells (ER-CALUX®) was applied to examine the ability of diesel exhaust to induce or inhibit estrogen receptor (ER)-mediated gene expression. Exhaust from a heavy-duty diesel engine was either treated by iron- or copper/iron-catalyzed diesel particulate filters (DPFs) or studied as unfiltered exhaust. Collected samples included particle-bound and semivolatile constituents of diesel exhaust. Our findings show that all of the samples contained compounds that were able to induce ER-mediated gene expression as well as compounds that suppressed the activity of the endogenous hormone 17β-estradiol (E2). Estrogenic activity prevailed over antiestrogenic activity. We found an overall ER-mediated activity of 1.63 ± 0.31 ng E2 CALUX equivalents (E2-CEQs) per m3 of unfiltered exhaust. In filtered exhaust, we measured 0.74 ± 0.07 (iron-catalyzed DPF) and 0.55 ± 0.09 ng E2-CEQ m−3 (copper/iron-catalyzed DPF), corresponding to reductions in estrogenic activity of 55 and 66%, respectively. Our study demonstrates that both catalytic DPFs lowered the ER-mediated endocrine-disrupting potential of diesel exhaust.
DOI 10.1007/s00216-008-1872-8
Additional Notes Received 26 September 2007, Revised 14 December 2007, Accepted 10 January 2008, Published online 9 February 2008
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03430
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NEBIS System Number 004239219
Source Database ID PP-35087
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  author = "Wenger, Daniela and Gerecke, Andreas C. and Heeb, Norbert V. and Naegeli, Hanspeter and Zenobi, Renato",
  title = "{C}atalytic diesel particulate filters reduce the in vitro estrogenic activity of diesel exhaust",
  journal = "Analytical and bioanalytical chemistry",
  year = 2008,
  volume = "390",
  number = "8",
  pages = "2021--2029",

E-Citations record created: Thu, 01 Apr 2010, 19:09:58 CET