Magnetically deliverable calcium phosphate nanoparticles for localized gene expression

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Author(s) Puddu, Michela, Broguiere, Nicolas, Mohn, Dirk, Zenobi-Wong, Marcy, Stark, Wendelin J., Grass, Robert N.
Publication Type Journal Items, Publication Status: Published
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Title Magnetically deliverable calcium phosphate nanoparticles for localized gene expression
Author(s) Puddu, Michela
Broguiere, Nicolas
Mohn, Dirk
Zenobi-Wong, Marcy
Stark, Wendelin J.
Grass, Robert N.
Journal or Series Title RSC Advances
Volume Number 5
Start Page 9997
End Page 10004
ISSN 2046-2069
Publisher Royal Society of Chemistry
Publication Place London
Publication Date 2015
Abstract Iron oxide doped tricalcium phosphate (Fe2O3@TCP) nanoparticles were designed as transfection vehicles and prepared by flame spray synthesis. Both components are known to be non-toxic and biocompatible. Calcium phosphate (CaP) facilitates DNA entry into cells without the need for toxic cationic mediators, while magnetic iron oxide allows for particle localization at a target site. Flame spray synthesis ensures easy and low-cost nanoparticle production in a reproducible way. Fe2O3@TCP nanoparticles, exhibiting DNA-binding capacity in the presence of CaCl2, were tested for transfection of a green fluorescent protein (GFP) encoding plasmid with Human Embryonic Kidney 293 (HEK 293) cells. Commercial magnetic agents, polyethylenimine (PEI) and standard calcium phosphate-mediated transfection were used for comparison. Transfection efficiency was estimated by GFP expression detected by fluorescence microscopy, while hoechst/ethidium homodimer-1 staining allowed the evaluation of method toxicity. We were able to efficiently transfect HEK 293 cells, and showed that Fe2O3@TCP particles and bound DNA can be concentrated in specific sites in a culture plate through the application of a magnetic field gradient to achieve localized transfection. While the commercial magnetic controls strongly affected cell growth and morphology, Fe2O3@TCP particles did not show marked toxicity and had only limited effects on cell proliferation. Overall performance in terms of transfection efficiency, cell proliferation and viability, were comparable to that of CaP and PEI, which lack magnetic targeting capability. The newly synthetized Fe2O3@TCP are, therefore, improved tools to deliver nucleic acids into cells and achieve spatial control of transfection.
DOI 10.1039/C4RA13413C
Additional Notes Received: 29 October 2014; Accepted: 5 January 2015
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03949
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NEBIS System Number 006752154
Source Database ID FORM-1421057932
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  author = "Puddu, Michela and Broguiere, Nicolas and Mohn, Dirk and Zenobi-Wong, Marcy and Stark, Wendelin J. and Grass, Robert N.",
  title = "{M}agnetically deliverable calcium phosphate nanoparticles for localized gene expression",
  journal = "RSC Advances",
  year = 2015,
  volume = "5",
  pages = "9997--10004",

E-Citations record created: Mon, 12 Jan 2015, 10:22:25 CET