Rapid and Precise Measurements of Gas-Phase Basicity of Peptides and Proteins at Atmospheric Pressure by Electrosonic Spray Ionization-Mass Spectrometry

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Author(s) Touboul, David, Jecklin, Matthias C., Zenobi, Renato
Publication Type Journal Items, Publication Status: Published
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Title Rapid and Precise Measurements of Gas-Phase Basicity of Peptides and Proteins at Atmospheric Pressure by Electrosonic Spray Ionization-Mass Spectrometry
Author(s) Touboul, David
Jecklin, Matthias C.
Zenobi, Renato
Journal or Series Title The journal of physical chemistry. B
Volume Number 111
Issue Number 40
Start Page 11629
End Page 11631
ISSN 1520-6106
1089-5647
1520-5207
Publisher American Chemical Society
Publication Place Washington, DC
Publication Date 2007
Abstract Deprotonation reactions of peptide and protein ions have been studied by introducing volatile reference bases at atmospheric pressure between an electrosonic spray ionization (ESSI) source and the inlet of a hybrid quadrupole time-of-flight mass spectrometer. This new setup offers the unique possibility to measure the apparent gas-phase basicity GBapp of multiply charged ions by a bracketing approach. A very good agreement has been obtained with reference values obtained by Fourier transform-ion cyclotronic resonance (FT-ICR), validating our approach. The measurements were then extended to larger biomolecules such as insulin and myoglobin in native and denaturing buffers. The main advantages of this methodology are measurements at atmospheric pressure with good sensitivity (for concentrations less than 10 μM in denaturing or nondenaturing buffer), very good precision (less than 2%), and in a short time (less than 30 min to screen up to 23 volatile reference bases).
DOI 10.1021/jp075127q
Additional Notes Received 2 July 2007, Revised 23 August 2007
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03430
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NEBIS System Number 001807682
Source Database ID PP-34147
WOS-000249986900006
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@article{Tbl2007,
  author = "Touboul, David and Jecklin, Matthias C. and Zenobi, Renato",
  title = "{R}apid and {P}recise {M}easurements of {G}as-{P}hase {B}asicity of {P}eptides and {P}roteins at {A}tmospheric {P}ressure by {E}lectrosonic {S}pray {I}onization-{M}ass {S}pectrometry",
  journal = "The journal of physical chemistry. B",
  year = 2007,
  volume = "111",
  number = "40",
  pages = "11629--11631",
}


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