Neutral desorption sampling coupled to extractive electrospray ionization mass spectrometry for rapid differentiation of biosamples by metabolomic fingerprinting

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Author(s) Chen, Huanwen, Wortmann, Arno, Zenobi, Renato
Publication Type Journal Items, Publication Status: Published
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Title Neutral desorption sampling coupled to extractive electrospray ionization mass spectrometry for rapid differentiation of biosamples by metabolomic fingerprinting
Author(s) Chen, Huanwen
Wortmann, Arno
Zenobi, Renato
Journal or Series Title Journal of mass spectrometry
Volume Number 42
Issue Number 9
Start Page 1123
End Page 1135
ISSN 1076-5174
1076-5164
1096-9888
Publisher Wiley
Publication Place Chichester
Publication Date 2007
Keyword(s) neutral desorption sampling
extractive electrospray ionization
biological samples
mass spectrometry
in vivo analysis
skin analysis
frozen meat analysis
spinach
E. coli
differential metabolomics
principal component analysis
Abstract It is of increasing interest and practical importance to develop convenient methods based on mass spectrometry for high-throughput analyses of biological samples. This is usually difficult because of the complex matrix and ion suppression effects. Generation of ions at ambient conditions is a promising solution to these problems because the sample is easily accessible and the ion suppression effect is reduced significantly. A new method for rapid on-line detection of metabolic markers in complex biological samples is described here. It combines atmospheric pressure desorption sampling by a gentle stream of air or nitrogen with extractive electrospray ionization (EESI) and mass spectrometric analysis. The resulting mass spectral fingerprints are shown to be able to detect spoilage of meat even in the frozen (-20 degrees C) state and the contamination of spinach by E. coli, and to identify metabolites and contaminants on human skin within seconds, in an on-line and high-throughput fashion. Typical molecular markers are identified using MS/MS data and by comparison with reference compounds. Differences between closely related samples are easily visualized by using principal component analysis (PCA) of the mass spectra data. The detection limit achieved is 10 fg/cm(2) (S/N = 3) for histamine on the surface of frozen meat. The technique reported here shows potential for more advanced applications in multiple disciplines, including food regulation, homeland security, in vivo metabolomics, and clinical diagnosis.
DOI 10.1002/jms.1282
Additional Notes Received 30 April 2007, Accepted 12 July 2007, Published Online 22 August 2007
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03430
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NEBIS System Number 001410098
Source Database ID PP-34138
WOS-000249463000001
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@article{Chn2007,
  author = "Chen, Huanwen and Wortmann, Arno and Zenobi, Renato",
  title = "{N}eutral desorption sampling coupled to extractive electrospray ionization mass spectrometry for rapid differentiation of biosamples by metabolomic fingerprinting",
  journal = "Journal of mass spectrometry",
  year = 2007,
  volume = "42",
  number = "9",
  pages = "1123--1135",
}


E-Citations record created: Thu, 01 Apr 2010, 18:34:35 CET