Investigation of the first shot phenomenon in MALDI mass spectrometry of protein complexes

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Author(s) Wortmann, Arno, Pimenova, Tatiana, Alves, Sandra, Zenobi, Renato
Publication Type Journal Items, Publication Status: Published
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Title Investigation of the first shot phenomenon in MALDI mass spectrometry of protein complexes
Author(s) Wortmann, Arno
Pimenova, Tatiana
Alves, Sandra
Zenobi, Renato
Journal or Series Title The Analyst
Volume Number 132
Issue Number 3
Start Page 199
End Page 207
ISSN 0003-2654
1364-5528
Publisher The Royal Society of Chemistry
Publication Place Cambridge
Publication Date 2007-00-00
Abstract Matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) of noncovalent protein complexes is difficult, due to the disruptive nature of processes occurring during MALDI sample preparation and ion formation. Sometimes the observation of intact noncovalent protein complexes with MALDI is only possible if data are acquired from the first laser shot fired at a fresh sample; this is called the 'first shot phenomenon'. To study the origin of the first shot phenomenon, we used MALDI-MS and confocal laser scanning microscopy (CLSM) to examine typical MALDI sample preparations with embedded protein complexes, labeled with fluorophores. Fluorescence energy transfer techniques allowed the differentiation between intact and dissociated protein complexes with CLSM. In cases where a first shot behavior was observed by MALDI-MS, it was found to be accompanied by localization of protein complexes at the exterior of the sample crystals. Segregation of the large protein complexes to the exterior and dissociation of the complexes in the crystal interior during sample crystallization can rationalize this observation.
DOI 10.1039/b615411e
Additional Notes Received 24 October 2006, Accepted 10 January 2007, Published online 18 January 2007
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03430
Organisational Unit(s)
NEBIS System Number 000023536
Source Database ID PP-34133
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@article{Wrtmnn2007,
  author = "Wortmann, Arno and Pimenova, Tatiana and Alves, Sandra and Zenobi, Renato",
  title = "{I}nvestigation of the first shot phenomenon in {M}{A}{L}{D}{I} mass spectrometry of protein complexes",
  journal = "The Analyst",
  year = 2007,
  volume = "132",
  number = "3",
  pages = "199--207",
}


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