Proteomic Profiling of a Snake Venom Using High Mass Detection MALDI-TOF Mass Spectrometry

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Author(s) Yanes, Oscar, Avilés, Francesc X., Wenzel, Ryan, Nazabal, Alexis, Calvete, Juan J., Zenobi, Renato
Publication Type Journal Items, Publication Status: Published
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Title Proteomic Profiling of a Snake Venom Using High Mass Detection MALDI-TOF Mass Spectrometry
Author(s) Yanes, Oscar
Avilés, Francesc X.
Wenzel, Ryan
Nazabal, Alexis
Calvete, Juan J.
Zenobi, Renato
Journal or Series Title Journal of the American Society for Mass Spectrometry
Volume Number 18
Issue Number 4
Start Page 600
End Page 606
ISSN 1044-0305
Publisher Elsevier Science
Publication Place New York
Publication Date 2007
Abstract Proteomic profiling involves identification and quantification of protein components in complex biological systems. Most of the mass profiling studies performed with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) have been restricted to peptides and small proteins (<20 kDa) because the sensitivity of the standard ion detectors decreases with increasing ion mass. Here we perform a protein profiling study of the snake venom Sistrurus miliarius barbouri, comparing 2D gel electrophoresis and reversed-phase high-performance liquid chromatography (HPLC) with a high mass cryodetector MALDI-TOF instrument (Macromizer), whose detector displays an uniform sensitivity with mass. Our results show that such MS approach can render superior analysis of protein complexity compared with that obtained with the electrophoretic and chromatographic approaches. The summation of ion impacts allows relative quantification of different proteins, and the number of ion counts correlates with the peak areas in the reversed-phase HPLC. Furthermore, the sensitivity reached with the high mass cryodetection MS technology clearly exceeds the detection limit of standard high-sensitivity staining methods.
DOI 10.1016/j.jasms.2006.11.007
Additional Notes Received 24 May 2006, Revised 23 October 2006, Accepted 7 November 2006, Available online 3 January 2007
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03430
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NEBIS System Number 000541631
Source Database ID PP-34130
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  author = "Yanes, Oscar and Avil{\'{e}}s, Francesc X. and Wenzel, Ryan and Nazabal, Alexis and Calvete, Juan J. and Zenobi, Renato",
  title = "{P}roteomic {P}rofiling of a {S}nake {V}enom {U}sing {H}igh {M}ass {D}etection {M}{A}{L}{D}{I}-{T}{O}{F} {M}ass {S}pectrometry",
  journal = "Journal of the American Society for Mass Spectrometry",
  year = 2007,
  volume = "18",
  number = "4",
  pages = "600--606",

E-Citations record created: Thu, 01 Apr 2010, 18:34:14 CET