Shrinking Droplets in Electrospray Ionization and Their Influence on Chemical Equilibria

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Author(s) Wortmann, Arno, Kistler-Momotova, Anna, Zenobi, Renato, Heine, Martin C., Wilhelm, Oliver, Pratsinis, Sotiris E.
Publication Type Journal Items, Publication Status: Published
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Title Shrinking Droplets in Electrospray Ionization and Their Influence on Chemical Equilibria
Author(s) Wortmann, Arno
Kistler-Momotova, Anna
Zenobi, Renato
Heine, Martin C.
Wilhelm, Oliver
Pratsinis, Sotiris E.
Journal or Series Title Journal of the American Society for Mass Spectrometry
Volume Number 18
Issue Number 3
Start Page 385
End Page 393
ISSN 1044-0305
Publisher Elsevier Science
Publication Place New York
Publication Date 2007
Abstract We investigated how chemical equilibria are affected by the electrospray process, using simultaneous in situ measurements by laser-induced fluorescence (LIF) and phase Doppler anemometry (PDA). The motivation for this study was the increasing number of publications in which electrospray ionization mass spectrometry is used for binding constant determination. The PDA was used to monitor droplet size and velocity, whereas LIF was used to monitor fluorescent analytes within the electrospray droplets. Using acetonitrile as solvent, we found an average initial droplet diameter of 10 μm in the electrospray. The PDA allowed us to follow the evolution of these droplets down to a size of 1 μm. Rhodamine B–sulfonylchloride was used as a fluorescent analyte within the electrospray. By spatially resolved LIF it was possible to probe the dimerization equilibrium of this dye. Measurements at different spray positions showed no influence of the decreasing droplet size on the monomer–dimer equilibrium. However, with the fluorescent dye pair DCM and oxazine 1 it was shown that a concentration increase does occur within electrosprayed droplets, using fluorescence resonance energy transfer as a probe for the average pair distance.
DOI 10.1016/j.jasms.2006.10.010
Additional Notes Received 3 May 2006, Revised 3 October 2006, Accepted 11 October 2006, Available online 16 November 2006
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03510
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NEBIS System Number 000541631
Source Database ID PP-34129
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  author = "Wortmann, Arno and Kistler-Momotova, Anna and Zenobi, Renato and Heine, Martin C. and Wilhelm, Oliver and Pratsinis, Sotiris E.",
  title = "{S}hrinking {D}roplets in {E}lectrospray {I}onization and {T}heir {I}nfluence on {C}hemical {E}quilibria",
  journal = "Journal of the American Society for Mass Spectrometry",
  year = 2007,
  volume = "18",
  number = "3",
  pages = "385--393",

E-Citations record created: Thu, 01 Apr 2010, 18:34:11 CET