Quantification of Saquinavir from Lysates of Peripheral Blood Mononuclear Cells Using Microarrays and Standard MALDI-TOF-MS

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Author(s) Pabst, Martin, Fagerer, Stephan R., Köhling, Rudolf, Eyer, Klaus, Krismer, Jasmin, Jefimovs, Konstantins, Ibáñez, Alfredo J., Zenobi, Renato
Publication Type Journal Items, Publication Status: Published
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Title Quantification of Saquinavir from Lysates of Peripheral Blood Mononuclear Cells Using Microarrays and Standard MALDI-TOF-MS
Author(s) Pabst, Martin
Fagerer, Stephan R.
Köhling, Rudolf
Eyer, Klaus
Krismer, Jasmin
Jefimovs, Konstantins
Ibáñez, Alfredo J.
Zenobi, Renato
Journal or Series Title Journal of the American Society for Mass Spectrometry
Volume Number 25
Issue Number 6
Start Page 1083
End Page 1086
ISSN 1044-0305
1879-1123
Publisher Springer
Publication Place New York, NY
Publication Date 2014
Keyword(s) Quantitative MALDI-MS
Saquinavir
Cell lysate analysis
Peripheral
Blood mononuclear cells
Microarrays for mass spectrometry (MAMS)
Antiretroviral drugs
Abstract Drug monitoring is usually performed by liquid chromatography coupled with optical detection or electrospray ionization mass spectrometry. More recently, matrix-assisted laser desorption/ionization (MALDI) in combination with triple quadrupole or Fourier-transform (FT) mass analyzers has also been reported to allow accurate quantification. Here, we present a strategy that employs standard MALDI time-of-flight (TOF) mass spectrometry (MS) for the sensitive and accurate quantification of saquinavir from an extract of blood peripheral mononuclear cells. Unambiguous identification of saquinavir in the mass spectra was possible because of using internal mass calibration and by an overall low chemical noise in the low mass range. Exact mass determination of the constant background peaks of the cell extract, which were used for recalibration, was performed by an initial MALDI-FT-MS analysis. Fast and multiplexed sample analysis was enabled by microarray technology, which provided 10 replicates in the lower nL range for each sample in parallel lanes on a chip. In order to validate the method, we employed various statistical tests, such as confidence intervals for linear regressions, three quality control samples, and inverse confidence limits of the estimated concentration ratios.
DOI 10.1007/s13361-014-0875-2
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03430
Organisational Unit(s)
NEBIS System Number 000541631
Source Database ID WOS-000336025800022
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@article{Pbst2014,
  author = "Pabst, Martin and Fagerer, Stephan R. and K{\"{o}}hling, Rudolf and Eyer, Klaus and Krismer, Jasmin and Jefimovs, Konstantins and Ib{\'{a}}{\~{n}}ez, Alfredo J. and Zenobi, Renato",
  title = "{Q}uantification of {S}aquinavir from {L}ysates of {P}eripheral {B}lood {M}ononuclear {C}ells {U}sing {M}icroarrays and {S}tandard {M}{A}{L}{D}{I}-{T}{O}{F}-{M}{S}",
  journal = "Journal of the American Society for Mass Spectrometry",
  year = 2014,
  volume = "25",
  number = "6",
  pages = "1083--1086",
}


E-Citations record created: Mon, 16 Jun 2014, 07:07:33 CET