Chondrocyte Culture in 3D Alginate Sulfate Hydrogels Promotes, Proliferation While Maintaining Expression of Chondrogenic Markers

Metadata Label Value
Author(s) Mhanna, Rami, Aditya, Aditya, Palazzolo, Gemma, Vallmajo-Martin, Queralt, Becher, Jana, Schnabelrauch, Matthias, Zenobi-Wong, Marcy, Möller, Stephanie
Publication Type Journal Items, Publication Status: Published
Full Text Search SFX for a Full-Text version of this document
Import to Mendeley Log in to provide feedback

Detailed Information

Metadata Field Content
Title Chondrocyte Culture in 3D Alginate Sulfate Hydrogels Promotes, Proliferation While Maintaining Expression of Chondrogenic Markers
Author(s) Mhanna, Rami
Aditya, Aditya
Palazzolo, Gemma
Vallmajo-Martin, Queralt
Becher, Jana
Schnabelrauch, Matthias
Zenobi-Wong, Marcy
Möller, Stephanie
Journal or Series Title Tissue engineering Part A
Volume Number 20
Issue Number 9/10
Start Page 1454
End Page 1464
ISSN 1937-3341
1937-335X
Publisher New Rochelle, NY
Publication Place Mary Ann Liebert
Publication Date 2013-06
Abstract The loss of expression of chondrogenic markers during monolayer expansion remains a stumbling block for cell-based treatment of cartilage lesions. Here we introduce sulfated alginate hydrogels as a cartilage biomimetic biomaterial which induces cell proliferation while maintaining the chondrogenic phenotype of encapsulated chondrocytes. Hydroxyl groups of alginate were converted to sulfates by incubation with sulfur trioxide-pyridine complex (SO3/pyridine), yielding a sulfated material crosslinkable with calcium chloride. Passage 3 bovine chondrocytes were encapsulated in alginate and alginate sulfate hydrogels for up to 35 days. Cell proliferation was 5-fold higher in alginate sulfate compared to alginate (p=0.038). Blocking beta1 integrins in chondrocytes within alginate sulfate hydrogels significantly inhibited proliferation (p=0.002). Sulfated alginate increased the RhoA activity of chondrocytes compared to unmodified alginate, an increase which was blocked by 1 blocking antibodies (p=0.017). Expression and synthesis of type II collagen, type I collagen and proteoglycan was not significantly affected by the encapsulation material evidenced by qRT-PCR and immunohistochemistry. Alginate sulfate constructs showed an opaque appearance in culture whereas the unmodified alginate samples remained translucent. In conclusion, alginate sulfate provides a 3D microenvironment which promotes both chondrocyte proliferation and maintenance of the chondrogenic phenotype and represents an important advance for chondrocyte-based cartilage repair therapies providing a material in which the cell expansion can be done in situ.
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03949
Organisational Unit(s)
NEBIS System Number 005837030
Source Database ID FORM-1389870039
Description File Name MIME Type Size
No details could be found
There are no links available for this record.
This record has not been viewed during this period

@article{Mhnn2013,
  author = "Mhanna, Rami and Aditya, Aditya and Palazzolo, Gemma and Vallmajo-Martin, Queralt and Becher, Jana and Schnabelrauch, Matthias and Zenobi-Wong, Marcy and M{\"{o}}ller, Stephanie",
  title = "{C}hondrocyte {C}ulture in 3{D} {A}lginate {S}ulfate {H}ydrogels {P}romotes, {P}roliferation {W}hile {M}aintaining {E}xpression of {C}hondrogenic {M}arkers",
  journal = "Tissue engineering Part A",
  year = 2013,
  volume = "20",
  number = "9/10",
  pages = "1454--1464",
  month = jun,
}


E-Citations record created: Thu, 16 Jan 2014, 11:01:09 CET