GFOGER-Modified MMP-Sensitive Polyethylene Glycol (PEG) Hydrogels Induce Chondrogenic Differentiation of Human Mesenchymal Stem Cells

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Author(s) Mhanna, Rami, Öztürk, Ece, Vallmajo-Martin, Queralt, Millan, Christopher, Müller, Michael, Zenobi-Wong, Marcy
Publication Type Journal Items, Publication Status: Published
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Title GFOGER-Modified MMP-Sensitive Polyethylene Glycol (PEG) Hydrogels Induce Chondrogenic Differentiation of Human Mesenchymal Stem Cells
Author(s) Mhanna, Rami
Öztürk, Ece
Vallmajo-Martin, Queralt
Millan, Christopher
Müller, Michael
Zenobi-Wong, Marcy
Journal or Series Title Tissue engineering. Part A
Volume Number 20
Issue Number 7-8
Start Page 1165
End Page 1174
ISSN 1937-3341
1937-335X
Publisher Mary Ann Liebert
Publication Place New Rochelle, NY
Publication Date 2014-04
Abstract The cellular microenvironment plays a crucial role in directing proliferation and differentiation of stem cells. Cells interact with their microenvironment via integrins which recognize certain peptide sequences of ECM proteins. This receptor-ligand binding has profound impact on cell fate. Interactions of human mesenchymal stem cells (hMSCs) with the triple helical collagen mimetic, GPC(GPP)5-GFOGER-(GPP)5GPC-NH2, and the fibronectin adhesion peptide, RGD, were studied in degradable or non-degradable PEG gels formed by Michael-type addition chemistry. Proliferation, cytoskeletal morphology, and chondrogenic differentiation of encapsulated hMSCs were evaluated. The hMSCs adopted a highly spread morphology within the GFOGER-modified gels, whereas RGD induced a star-like spreading of the cells. hMSCs within GFOGER-modified degradable gels had a high proliferation rate compared to cells in peptide free gels (p=0.017). Gene expression of type II collagen and aggrecan was highest in GFOGER-modified degradable gels after 7 and 21 days. Peptide incorporation increased GAG production in degradable gels after 7 and 21 days and GFOGER-modified degradable hydrogels had on average the highest GAG content, a finding which was confirmed by Alcian blue staining. In conclusion, the GFOGER peptide enhances proliferation in degradable PEG gels and provides a better chondrogenic microenvironment compared to the RGD peptide.
DOI 10.1089/ten.TEA.2013.0519
Additional Notes Received 19 August 2013, Accepted 19 August 2013, Published online 17 October 2013
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03949
Organisational Unit(s)
NEBIS System Number 005837030
Source Database ID FORM-1389868816
FORM-1421247244
SCOPUS-84898415623
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@article{Mhnn2014,
  author = "Mhanna, Rami and {\"{O}}zt{\"{u}}rk, Ece and Vallmajo-Martin, Queralt and Millan, Christopher and M{\"{u}}ller, Michael and Zenobi-Wong, Marcy",
  title = "{G}{F}{O}{G}{E}{R}-{M}odified {M}{M}{P}-{S}ensitive {P}olyethylene {G}lycol ({P}{E}{G}) {H}ydrogels {I}nduce {C}hondrogenic {D}ifferentiation of {H}uman {M}esenchymal {S}tem {C}ells",
  journal = "Tissue engineering. Part A",
  year = 2014,
  volume = "20",
  number = "7-8",
  pages = "1165--1174",
  month = apr,
}


E-Citations record created: Thu, 16 Jan 2014, 10:40:27 CET