GFOGER-Modified MMP-Sensitive Polyethylene Glycol (PEG) Hydrogels Induce Chondrogenic Differentiation of Human Mesenchymal Stem Cells

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Author(s) Mhanna, Rami, Öztürk, Ece, Vallmajo-Martin, Queralt, Millan, Christopher, Müller, Michael, Zenobi-Wong, Marcy
Publication Type Journal Items, Publication Status: Published
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Title GFOGER-Modified MMP-Sensitive Polyethylene Glycol (PEG) Hydrogels Induce Chondrogenic Differentiation of Human Mesenchymal Stem Cells
Author(s) Mhanna, Rami
Öztürk, Ece
Vallmajo-Martin, Queralt
Millan, Christopher
Müller, Michael
Zenobi-Wong, Marcy
Journal or Series Title Tissue engineering. Part A
Volume Number 20
Issue Number 7-8
Start Page 1165
End Page 1174
ISSN 1937-3341
Publisher Mary Ann Liebert
Publication Place New Rochelle, NY
Publication Date 2014-04
Abstract The cellular microenvironment plays a crucial role in directing proliferation and differentiation of stem cells. Cells interact with their microenvironment via integrins which recognize certain peptide sequences of ECM proteins. This receptor-ligand binding has profound impact on cell fate. Interactions of human mesenchymal stem cells (hMSCs) with the triple helical collagen mimetic, GPC(GPP)5-GFOGER-(GPP)5GPC-NH2, and the fibronectin adhesion peptide, RGD, were studied in degradable or non-degradable PEG gels formed by Michael-type addition chemistry. Proliferation, cytoskeletal morphology, and chondrogenic differentiation of encapsulated hMSCs were evaluated. The hMSCs adopted a highly spread morphology within the GFOGER-modified gels, whereas RGD induced a star-like spreading of the cells. hMSCs within GFOGER-modified degradable gels had a high proliferation rate compared to cells in peptide free gels (p=0.017). Gene expression of type II collagen and aggrecan was highest in GFOGER-modified degradable gels after 7 and 21 days. Peptide incorporation increased GAG production in degradable gels after 7 and 21 days and GFOGER-modified degradable hydrogels had on average the highest GAG content, a finding which was confirmed by Alcian blue staining. In conclusion, the GFOGER peptide enhances proliferation in degradable PEG gels and provides a better chondrogenic microenvironment compared to the RGD peptide.
DOI 10.1089/ten.TEA.2013.0519
Additional Notes Received 19 August 2013, Accepted 19 August 2013, Published online 17 October 2013
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03949
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NEBIS System Number 005837030
Source Database ID FORM-1389868816
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  author = "Mhanna, Rami and {\"{O}}zt{\"{u}}rk, Ece and Vallmajo-Martin, Queralt and Millan, Christopher and M{\"{u}}ller, Michael and Zenobi-Wong, Marcy",
  title = "{G}{F}{O}{G}{E}{R}-{M}odified {M}{M}{P}-{S}ensitive {P}olyethylene {G}lycol ({P}{E}{G}) {H}ydrogels {I}nduce {C}hondrogenic {D}ifferentiation of {H}uman {M}esenchymal {S}tem {C}ells",
  journal = "Tissue engineering. Part A",
  year = 2014,
  volume = "20",
  number = "7-8",
  pages = "1165--1174",
  month = apr,

E-Citations record created: Thu, 16 Jan 2014, 10:40:27 CET