Self-Aliquoting Microarray Plates for Accurate Quantitative Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

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Author(s) Pabst, Martin, Fagerer, Stephan R., Kohling, Rudolf, Kuster, Simon K., Steinhofft, Robert, Badertscher, Martin, Wahl, Fabian, Dittrich, Petra S., Jefimovs, Konstantins, Zenobi, Renato
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Title Self-Aliquoting Microarray Plates for Accurate Quantitative Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry
Author(s) Pabst, Martin
Fagerer, Stephan R.
Kohling, Rudolf
Kuster, Simon K.
Steinhofft, Robert
Badertscher, Martin
Wahl, Fabian
Dittrich, Petra S.
Jefimovs, Konstantins
Zenobi, Renato
Description 6p.
Journal or Series Title Analytical Chemistry
Volume Number 85
Issue Number 20
Start Page 9771
ISSN 0003-2700
Publisher American Chemical Society
Publication Place Washington, D.C
Publication Date 2013-09
Abstract Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is a fast analysis tool employed for the detection of a broad range of analytes. However, MALDI-MS has a reputation of not being suitable for quantitative analysis. Inhomogeneous analyte/matrix co-crystallization, spot-to-spot inhomogeneity, as well as a typically low number of replicates are the main contributing factors. Here, we present a novel MALDI sample target for quantitative MALDI-MS applications, which addresses the limitations mentioned above. The platform is based on the recently developed microarray for mass spectrometry (MAMS) technology and contains parallel lanes of hydrophilic reservoirs. Samples are not pipetted manually but deposited by dragging one or several sample droplets with a metal sliding device along these lanes. Sample is rapidly and automatically aliquoted into the sample spots due to the interplay of hydrophilic/hydrophobic interactions. With a few microliters of sample, it is possible to aliquot up to 40 replicates within seconds, each aliquot containing just 10 nL. The analyte droplet dries immediately and homogeneously, and consumption of the whole spot during MALDI-MS analysis is typically accomplished within few seconds. We evaluated these sample targets with respect to their suitability for use with different samples and matrices. Furthermore, we tested their application for generating calibration curves of standard peptides with alpha-cyano-4-hdydroxycinnamic acid as a matrix. For angiotensin II and [Glu(1)]-fibrinopeptide B we achieved coefficients of determination (r(2)) greater than 0.99 without the use of internal standards.
DOI 10.1021/ac4021775
Additional Notes Received 16 July 2013, Accepted 4 September 2013, Published 4 September 2013
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03807
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NEBIS System Number 000005465
Source Database ID WOS-000326126600050
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  author = "Pabst, Martin and Fagerer, Stephan R. and Kohling, Rudolf and Kuster, Simon K. and Steinhofft, Robert and Badertscher, Martin and Wahl, Fabian and Dittrich, Petra S. and Jefimovs, Konstantins and Zenobi, Renato",
  title = "{S}elf-{A}liquoting {M}icroarray {P}lates for {A}ccurate {Q}uantitative {M}atrix-{A}ssisted {L}aser {D}esorption/{I}onization {M}ass {S}pectrometry",
  journal = "Analytical Chemistry",
  year = 2013,
  volume = "85",
  number = "20",
  pages = "9771--",
  month = sep,

E-Citations record created: Mon, 25 Nov 2013, 07:46:55 CET