Analysis of single algal cells by combining mass spectrometry with Raman and fluorescence mapping

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Author(s) Fagerer, Stephan R., Schmid, Thomas, Ibáñez, Alfredo J., Pabst, Martin, Steinhoff, Robert, Jefimovs, Konstantins, Urbanc, Pawel L., Zenobi, Renato
Publication Type Journal Items, Publication Status: Published
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Title Analysis of single algal cells by combining mass spectrometry with Raman and fluorescence mapping
Author(s) Fagerer, Stephan R.
Schmid, Thomas
Ibáñez, Alfredo J.
Pabst, Martin
Steinhoff, Robert
Jefimovs, Konstantins
Urbanc, Pawel L.
Zenobi, Renato
Journal or Series Title Analyst
Volume Number 138
Issue Number 22
Start Page 6732
End Page 6736
ISSN 0003-2654
Publisher Royal Society of Chemistry
Publication Place Cambridge
Publication Date 2013-11
Abstract In order to investigate metabolic properties of single cells of freshwater algae (Haematococcus pluvialis), we implement matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) in combination with microspectroscopic mapping. Straightforward coupling of these two detection platforms was possible thanks to the self-aliquoting properties of micro-arrays for mass spectrometry (MAMS). Following Raman and fluorescence imaging, the isolated cells were covered with a MALDI matrix for targeted metabolic analysis by MALDI-MS. The three consecutive measurements carried out on the same cells yielded complementary information. Using this method, we were able to study the encystment of H. pluvialis – by monitoring the adenosine triphosphate (ATP) to adenosine diphosphate (ADP) ratio during the build-up of astaxanthin in the cells as well as the release of β-carotene, the precursor of astaxanthin, into the cytosol.
DOI 10.1039/c3an01135f
Additional Notes Received 7 June 2013, Accepted 20 August 2013, First published online 20 August 2013
Document Type Article
Publication Status Published
Language English
NEBIS System Number 000023536
Source Database ID SCOPUS-84885922545
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  author = "Fagerer, Stephan R. and Schmid, Thomas and Ib{\'{a}}{\~{n}}ez, Alfredo J. and Pabst, Martin and Steinhoff, Robert and Jefimovs, Konstantins and Urbanc, Pawel L. and Zenobi, Renato",
  title = "{A}nalysis of single algal cells by combining mass spectrometry with {R}aman and fluorescence mapping",
  journal = "Analyst",
  year = 2013,
  volume = "138",
  number = "22",
  pages = "6732--6736",
  month = nov,

E-Citations record created: Tue, 05 Nov 2013, 08:59:49 CET