Probing the microenvironmental conditions for induction of superficial zone protein expression

Metadata Label Value
Author(s) Mhanna, R., Öztürk, Ece, Schlink, P., Zenobi-Wong, Marcy
Publication Type Journal Items, Publication Status: Published
Full Text Search SFX for a Full-Text version of this document
Import to Mendeley Log in to provide feedback

Detailed Information

Metadata Field Content
Title Probing the microenvironmental conditions for induction of superficial zone protein expression
Author(s) Mhanna, R.
Öztürk, Ece
Schlink, P.
Zenobi-Wong, Marcy
Journal or Series Title Osteoarthritis and cartilage
Volume Number 21
Issue Number 12
Start Page 1924
End Page 1932
ISSN 1063-4584
1522-9653
Publisher Elsevier
Publication Place Amsterdam
Publication Date 2013-12
Keyword(s) Superficial zone protein
Chondrocytes dedifferentiation
Mechanical strain
Oxygen tension
Microenvironment
Abstract Objective: To determine the in vitro conditions which promote expression of superficial zone protein (SZP).
Methods: Chondrocytes from 6-month-old calves were expanded in monolayer culture and the expression of SZP in alginate bead and monolayer culture was quantified with quantitative real time-polymerase chain reaction (qRT-PCR) and immunostaining. The effect of oxygen tension on SZP expression was determined by qRT-PRC analysis of cells cultured in two dimension (2D) and three dimension (3D) under hypoxic (1% pO2) or normoxic (21% pO2) conditions. Finally, to examine the effect of cyclic tensile strain on expression of SZP in 2D and 3D cultures, chondrocytes encapsulated in alginate beams or seeded on type I collagen coated polydimethylsiloxane (PDMS) chambers were subjected to 5% strain at 1 Hz, 2 h/day for 4 days or 2 h at the fourth day of culture and mRNA levels were quantified.
Results: Bovine chondrocytes in monolayer showed a drastic decrease in SZP expression, similar in trend to the commonly reported downregulation of type II collagen (Col2). Chondrocytes embedded in alginate beads for 4 days re-expressed SZP but not Col2. SZP expression was higher under normoxic conditions whereas Col2 was upregulated only in alginate beads under hypoxic conditions. Cyclic mechanical strain showed a tendency to upregulate mRNA levels of SZP.
Conclusions: A microenvironment encompassing a soft encapsulation material and 21% oxygen is sufficient for fibroblastic chondrocytes to re-express SZP. These results serve as a guideline for the design of stratified engineered articular cartilage and suggest that microenvironmental cues (oxygen tension level) strongly influence the pattern of SZP expression in vivo.
DOI 10.1016/j.joca.2013.08.017
Additional Notes Received 24 January 2013, Accepted 14 August 2013
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03949
Organisational Unit(s)
NEBIS System Number 004530868
Source Database ID SCOPUS-84888870125
WOS-000329266700013
Description File Name MIME Type Size
No details could be found
There are no links available for this record.
This record has not been viewed during this period

@article{Mhnn2013,
  author = "Mhanna, R. and {\"{O}}zt{\"{u}}rk, Ece and Schlink, P. and Zenobi-Wong, Marcy",
  title = "{P}robing the microenvironmental conditions for induction of superficial zone protein expression",
  journal = "Osteoarthritis and cartilage",
  year = 2013,
  volume = "21",
  number = "12",
  pages = "1924--1932",
  month = dec,
}


E-Citations record created: Tue, 01 Oct 2013, 08:43:12 CET