Mass Discrimination in High-Mass MALDI-MS

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Author(s) Weidmann, Simon, Mikutis, Gediminas, Barylyuk, Konstantin, Zenobi, Renato
Publication Type Journal Items, Publication Status: Published
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Title Mass Discrimination in High-Mass MALDI-MS
Author(s) Weidmann, Simon
Mikutis, Gediminas
Barylyuk, Konstantin
Zenobi, Renato
Journal or Series Title Journal of the American Society for Mass Spectrometry
Volume Number 24
Issue Number 9
Start Page 1396
End Page 1404
ISSN 1044-0305
Publisher Springer
Publication Place New York, NY, USA
Publication Date 2013
Keyword(s) Relative response factor
Mass discrimination
Protein analysis
Ion conversion dynodes
Abstract In high-mass matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), the accessible m/z range is limited by the detector used. Therefore, special high-mass detectors based on ion conversion dynodes (ICDs) have been developed. Recently, we have found that mass bias may exist when such ICD detectors are used [Weidmann et al., Anal. Chem. 85(6), 3425-3432 (2013)]. In this contribution, the mass-dependent response of an ICD detector was systematically studied, the response factors for proteins with molecular weights from 35.9 to 129.9 kDa were determined, and the reasons for mass bias were identified. Compared with commonly employed microchannel plate detectors, we found that the mass discrimination is less pronounced, although ions with higher masses are weakly favored when using an ICD detector. The relative response was found to depend on the laser power used for MALDI; low-mass ions are discriminated against with higher laser power. The effect of mutual ion suppression in dependence of the proteins used and their molar ratio is shown. Mixtures consisting of protein oligomers that only differ in mass show less mass discrimination than mixtures consisting of different proteins with similar masses. Furthermore, mass discrimination increases for molar ratios far from 1. Finally, we present clear guidelines that help to choose the experimental parameters such that the response measured matches the actual molar fraction as closely as possible.
DOI 10.1007/s13361-013-0686-x
Document Type Article
Publication Status Published
Language English
NEBIS System Number 000541631
Source Database ID WOS-000323060000011
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  author = "Weidmann, Simon and Mikutis, Gediminas and Barylyuk, Konstantin and Zenobi, Renato",
  title = "{M}ass {D}iscrimination in {H}igh-{M}ass {M}{A}{L}{D}{I}-{M}{S}",
  journal = "Journal of the American Society for Mass Spectrometry",
  year = 2013,
  volume = "24",
  number = "9",
  pages = "1396--1404",

E-Citations record created: Mon, 16 Sep 2013, 09:13:13 CET