Mass spectrometry-based metabolomics of single yeast cells

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Author(s) Ibaneza, Alfredo J., Fagerer, Stephan R., Schmidt, Anna Mareike, Urban, Pawel L., Jefimovs, Konstantins, Geiger, Philipp, Dechant, Reinhard, Heinemann, Matthias, Zenobi, Renato
Publication Type Journal Items, Publication Status: Published
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Title Mass spectrometry-based metabolomics of single yeast cells
Author(s) Ibaneza, Alfredo J.
Fagerer, Stephan R.
Schmidt, Anna Mareike
Urban, Pawel L.
Jefimovs, Konstantins
Geiger, Philipp
Dechant, Reinhard
Heinemann, Matthias
Zenobi, Renato
Journal or Series Title Proceedings of the National Academy of Sciences of the United States of America
Volume Number 110
Issue Number 22
Start Page 8790
End Page 8794
ISSN 0027-8424
1091-6490
Publisher National Academy of Sciences
Publication Place Washington, D.C.
Publication Date 2013-05
Keyword(s) Single-cell measurements
MALDI mass spectrometry
Baker's yeast
Abstract Single-cell level measurements are necessary to characterize the intrinsic biological variability in a population of cells. In this study, we demonstrate that, with the microarrays for mass spectrometry platform, we are able to observe this variability. We monitor environmentally (2-deoxy-D-glucose) and genetically (Delta PFK2) perturbed Saccharomyces cerevisiae cells at the single-cell, few-cell, and population levels. Correlation plots between metabolites from the glycolytic pathway, as well as with the observed ATP/ADP ratio as a measure of cellular energy charge, give biological insight that is not accessible from population-level metabolomic data.
DOI 10.1073/pnas.1209302110
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03595
03430
Organisational Unit(s)
NEBIS System Number 001003347
Source Database ID WOS-000320500000028
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@article{bnz2013,
  author = "Ibaneza, Alfredo J. and Fagerer, Stephan R. and Schmidt, Anna Mareike and Urban, Pawel L. and Jefimovs, Konstantins and Geiger, Philipp and Dechant, Reinhard and Heinemann, Matthias and Zenobi, Renato",
  title = "{M}ass spectrometry-based metabolomics of single yeast cells",
  journal = "Proceedings of the National Academy of Sciences of the United States of America",
  year = 2013,
  volume = "110",
  number = "22",
  pages = "8790--8794",
  month = may,
}


E-Citations record created: Mon, 12 Aug 2013, 09:48:19 CET