Matrix-assisted laser desorption/ionization matrices for negative mode metabolomics

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Author(s) Fagerer, Stephan R., Nielsen, Simone, Ibanez, Alfredo, Zenobi, Renato
Publication Type Journal Items, Publication Status: Published
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Title Matrix-assisted laser desorption/ionization matrices for negative mode metabolomics
Author(s) Fagerer, Stephan R.
Nielsen, Simone
Ibanez, Alfredo
Zenobi, Renato
Journal or Series Title European journal of mass spectrometry
Volume Number 19
Issue Number 1
Start Page 39
End Page 47
ISSN 1469-0667
Publisher IM Publications
Publication Place Chichester
Publication Date 2013
Keyword(s) Metabolomics
Negative mode MALDI
MALDI matrix screening
Abstract Matrix-assisted laser desorption/ionization (MALDI) has been shown to be highly sensitive for analyzing low-mass compounds such as metabolites if the right matrix is used. 9-aminoacridine (9AA) is the most commonly employed matrix for negative mode MALDI-MS in metabolomics. However, matrix interferences and the strongly varying sensitivity for different metabolites make a search for alternative matrices desirable, in order to identify compounds with a different chemical background and/or favoring a different range of analytes. We tested the performance of a series of potential negative mode MALDI matrices with a mix of 29 metabolites containing amino acids, nucleotide phosphates and Krebs cycle intermediates. While ethacridine lactate was found to provide Limits of detection (LODs) in the low femtomole range for nucleotide phosphates, amino acids and Krebs cycle intermediates in the low picomole range, 4-amino-2-methylquinoline showed LODs in the picomole range for most metabolites, but is capable of ionizing a broader range of analytes than both 9AA and ethacridine.
DOI 10.1255/ejms.1209
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03430
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Source Database ID WOS-000319033800004
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  author = "Fagerer, Stephan R. and Nielsen, Simone and Ibanez, Alfredo and Zenobi, Renato",
  title = "{M}atrix-assisted laser desorption/ionization matrices for negative mode metabolomics",
  journal = "European journal of mass spectrometry",
  year = 2013,
  volume = "19",
  number = "1",
  pages = "39--47",

E-Citations record created: Mon, 24 Jun 2013, 06:18:54 CET