Immunoassays with direct mass spectrometric detection

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Author(s) Nazabal, Alexis, Wenzel, Ryan J., Zenobi, Renato
Publication Type Journal Items, Publication Status: Published
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Title Immunoassays with direct mass spectrometric detection
Author(s) Nazabal, Alexis
Wenzel, Ryan J.
Zenobi, Renato
Journal or Series Title Analytical chemistry
Volume Number 78
Issue Number 11
Start Page 3562
End Page 3570
ISSN 0003-2700
Publisher American Chemical Society
Publication Place Washington, DC
Publication Date 2006-06
Abstract A rapid, specific, and sensitive method for the detection of protein-protein interactions is of crucial importance for drug discovery and clinical diagnostics. Mass spectrometry plays a major role in the analysis of proteins, but its application to the routine analysis of protein complexes has been lagging behind. A new strategy for high-throughput analysis of protein interactions is presented here. We demonstrate application to immunochemical questions such as epitope mapping, kinetic studies, and sandwich assays. The methodology is based on a direct mass spectrometric readout for antigen-antibody complexes in the 150-400 kDa range. This has become possible using a novel detector technology and chemical cross-linking to stabilize complexes for analysis by MALDI MS. We demonstrate high detection sensitivity ( femtomole quantities of antigen), high specificity ( specific detection of antigen directly in serum), high accuracy, and high speed ( minutes per assay), surpassing conventional analytical methods by more than 2 orders of magnitude.
DOI 10.1021/ac0519108
Additional Notes Received 26 October 2005, Accepted 27 March 2006, Published online 6 May 2006
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03430
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NEBIS System Number 000005465
Source Database ID PP-24959
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  author = "Nazabal, Alexis and Wenzel, Ryan J. and Zenobi, Renato",
  title = "{I}mmunoassays with direct mass spectrometric detection",
  journal = "Analytical chemistry",
  year = 2006,
  volume = "78",
  number = "11",
  pages = "3562--3570",
  month = jun,

E-Citations record created: Thu, 01 Apr 2010, 16:14:52 CET