A New, Modular Mass Calibrant for High-Mass MALDI-MS

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Author(s) Weidmann, Simon, Barylyuk, Konstantin, Nespovitaya, Nadezhda, Madler, Stefanie, Zenobi, Renato
Publication Type Journal Items, Publication Status: Published
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Title A New, Modular Mass Calibrant for High-Mass MALDI-MS
Author(s) Weidmann, Simon
Barylyuk, Konstantin
Nespovitaya, Nadezhda
Madler, Stefanie
Zenobi, Renato
Journal or Series Title Analytical chemistry
Volume Number 85
Issue Number 6
Start Page 3425
End Page 3432
ISSN 0003-2700
Publisher American Chemical Society
Publication Place Washington, DC
Publication Date 2013-03-19
Abstract The application of matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) for the analysis of high-mass proteins requires suitable calibration standards at high m/z ratios. Several possible candidates were investigated, and concatenated polyproteins based on recombinantly expressed maltodextrin-binding protein (MBP) are shown here to be well-suited for this purpose. Introduction of two specific recognition sites into the primary sequence of the polyprotein allows for the selective cleavage of MBP3 into MBP and MBP2. Moreover, these MBP2 and MBP3 oligomers can be dimerized specifically, such that generation of MPB4 and MBP6 is possible as well. With the set of calibrants presented here, the m/z range of 40-400 kDa is covered. Since all calibrants consist of the same species and differ only in mass, the ionization efficiency is expected to be similar. However, equimolar mixtures of these proteins did not yield equal signal intensities on a detector specifically designed for detecting high-mass molecules.
DOI 10.1021/ac400129h
Additional Notes Received 14 January 2013, Accepted 8 February 2013
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03430
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NEBIS System Number 000005465
Source Database ID WOS-000316520500053
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  author = "Weidmann, Simon and Barylyuk, Konstantin and Nespovitaya, Nadezhda and Madler, Stefanie and Zenobi, Renato",
  title = "{A} {N}ew, {M}odular {M}ass {C}alibrant for {H}igh-{M}ass {M}{A}{L}{D}{I}-{M}{S}",
  journal = "Analytical chemistry",
  year = 2013,
  volume = "85",
  number = "6",
  pages = "3425--3432",
  month = mar,

E-Citations record created: Mon, 29 Apr 2013, 07:07:12 CET