Native Biomolecules in the Gas Phase?

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Author(s) Frankevich, Vladimir, Barylyuk, Konstantin, Chingin, Konstantin, Nieckarz, Robert, Zenobi, Renato
Publication Type Journal Items, Publication Status: Published
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Title Native Biomolecules in the Gas Phase?
Subtitle The Case of Green Fluorescent Protein
Author(s) Frankevich, Vladimir
Barylyuk, Konstantin
Chingin, Konstantin
Nieckarz, Robert
Zenobi, Renato
Journal or Series Title ChemPhysChem
Volume Number 14
Issue Number 5
Start Page 929
End Page 935
ISSN 1439-4235
1439-7641
Publisher Wiley
Publication Place Weinheim
Publication Date 2013-04-02
Keyword(s) Biomolecules
Conformational change
Fluorescence
Green fluorescent protein
Mass spectrometry
Abstract Green fluorescent protein (GFP) was ionized by native electrospray ionization and trapped for many seconds in high vacuum, allowing fluorescence emission to be measured as a probe of its biological function, to answer the question whether GFP exists in the native form in the gas phase or not. Although a narrow charge-state distribution, a collision cross-section very close to that expected for correctly folded GFP, and a large stability against dissociation all support a near-native gas-phase structure, no fluorescence emission was observed. The loss of the native form is attributed to the absence of residual water in the gas phase, which normally stabilizes the para-hydroxybenzylidene imidazolone chromophore of GFP.
DOI 10.1002/cphc.201200959
Additional Notes Received 20 November 2012, Revised 22 January 2013, Published online 21 February 2013
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03430
Organisational Unit(s)
NEBIS System Number 003846352
Source Database ID WOS-000316624600010
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@article{Frnkvch2013,
  author = "Frankevich, Vladimir and Barylyuk, Konstantin and Chingin, Konstantin and Nieckarz, Robert and Zenobi, Renato",
  title = "{N}ative {B}iomolecules in the {G}as {P}hase?: {T}he {C}ase of {G}reen {F}luorescent {P}rotein",
  journal = "ChemPhysChem",
  year = 2013,
  volume = "14",
  number = "5",
  pages = "929--935",
  month = apr,
}


E-Citations record created: Mon, 29 Apr 2013, 06:43:11 CET