Full Spectroscopic Tip-Enhanced Raman Imaging of Single Nanotapes Formed from β-Amyloid(1–40) Peptide Fragments

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Author(s) Paulite, Melissa, Blum, Carolin, Schmid, Thomas, Opilik, Lothar, Eyer, Klaus, Walker, Gilbert C., Zenobi, Renato
Publication Type Journal Items, Publication Status: Published
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Title Full Spectroscopic Tip-Enhanced Raman Imaging of Single Nanotapes Formed from β-Amyloid(1–40) Peptide Fragments
Author(s) Paulite, Melissa
Blum, Carolin
Schmid, Thomas
Opilik, Lothar
Eyer, Klaus
Walker, Gilbert C.
Zenobi, Renato
Journal or Series Title ACS nano
Volume Number 7
Issue Number 2
Start Page 911
End Page 920
ISSN 1936-0851
1936-086X
Publisher American Chemical Society
Publication Place Columbus, OH
Publication Date 2013-02
Keyword(s) Tip-enhanced Raman spectroscopy
TERS
β-amyloid nanotapes
Imaging
Marker band
Abstract This study demonstrates that spectral fingerprint patterns for a weakly scattering biological sample can be obtained reproducibly and reliably with tip-enhanced Raman spectroscopy (TERS) that correspond well with the conventional confocal Raman spectra collected for the bulk substance. These provided the basis for obtaining TERS images of individual self-assembled peptide nanotapes using an automated, objective procedure that correlate with the simultaneously obtained scanning tunneling microscopy (STM) images. TERS and STM images (64 x 64 pixels, 3 x 3 mu m(2)) of peptide nanotapes are presented that rely on marker bands in the Raman fingerprint region. Full spectroscopic information in every pixel was obtained, allowing post-processing of data and identification of species of interest. Experimentally, the "gap-mode" TERS configuration was used with a solid metal (Ag) tip in feedback with a metal substrate (Au). Confocal Raman data of bulk nanotapes, TERS point measurements with longer acquisition time, atomic force microscopy images, and an infrared absorption spectrum of bulk nanotapes were recorded for comparison. It is shown that the unique combination of topographic and spectroscopic data that TERS imaging provides reveals differences between the STM and TERS images, for example, nanotapes that are only weakly visible in the STM images, a coverage of the surface with an unknown substance, and the identification of a patch as a protein assembly that could not be unambiguously assigned based on the STM image alone.
DOI 10.1021/nn305677k
Additional Notes Just accepted manuscript 14 January 2013, Received 24 October 2012, Accepted 13 January 2013
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03430
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NEBIS System Number 005422099
Source Database ID WOS-000315618700007
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@article{Plt2013,
  author = "Paulite, Melissa and Blum, Carolin and Schmid, Thomas and Opilik, Lothar and Eyer, Klaus and Walker, Gilbert C. and Zenobi, Renato",
  title = "{F}ull {S}pectroscopic {T}ip-{E}nhanced {R}aman {I}maging of {S}ingle {N}anotapes {F}ormed from β-{A}myloid(1{\textendash}40) {P}eptide {F}ragments",
  journal = "ACS nano",
  year = 2013,
  volume = "7",
  number = "2",
  pages = "911--920",
  month = feb,
}


E-Citations record created: Mon, 08 Apr 2013, 07:57:09 CET