Interfacing droplet microfluidics with matrix-assisted laser desorption/ionization mass spectrometry

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Author(s) Kuster, Simon K., Fagerer, Stephan R., Verboket, Pascal E., Eyer, Klaus, Jefimovs, Konstantins, Zenobi, Renato, Dittrich, Petra S.
Publication Type Journal Items, Publication Status: Published
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Title Interfacing droplet microfluidics with matrix-assisted laser desorption/ionization mass spectrometry
Subtitle Label-Free Content Analysis of Single Droplets
Author(s) Kuster, Simon K.
Fagerer, Stephan R.
Verboket, Pascal E.
Eyer, Klaus
Jefimovs, Konstantins
Zenobi, Renato
Dittrich, Petra S.
Journal or Series Title Analytical Chemistry
Volume Number 85
Issue Number 3
Start Page 1285
End Page 1289
ISSN 0003-2700
Publisher American Chemical Society
Publication Place Washington, DC
Publication Date 2013-02
Abstract Droplet-based microfluidic systems have become a very powerful tool to miniaturize chemical and biological reactions. However, droplet content analysis remains challenging and relies almost exclusively on optical methods such as fluorescence spectroscopy. Hence, labeling of the analyte is typically required which impedes a more universal applicability of microdroplets. Here we present a novel interface coupling droplet microfluidics and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry for label-free content analysis of single droplets. Nano liter aqueous droplets immersed in perfluorinated oil are created in a microfluidic T-junction, transferred into a capillary, and deposited on a high-density microarray MALDI plate mounted on a motorized xy-stage. The fully automated system is robust and reliable due to two unique features. First, a simple optical droplet detection system is used to synchronize stage movement and exit of droplets from the capillary. Second, the microarray plate contains an array of over 26 000 hydrophilic spots within a hydrophobic coating, each spot acting as a recipient to confine the droplets and to prevent cross-contamination. The MALDI matrix can also be applied using our system by spotting matrix droplets on the microarray in a separate run. To demonstrate the potential of our system, we studied the enzymatic cleavage of angiotensin I by angiotensin converting enzyme and monitored the increasing concentration of the product angiotensin II over time. The interface provides a robust and fully automated method for rapid label-free and information-rich content analysis of single droplets. With the high number of droplets per plate, this method is particularly suitable for high-throughput screening applications.
DOI 10.1021/ac3033189
Additional Notes Received 15 November 2012, Accepted 5 January 2013, Published online 5 January 2013
Document Type Article
Publication Status Published
Language English
Assigned Organisational Unit(s) 03430
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NEBIS System Number 000005465
Source Database ID WOS-000314676100007
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  author = "Kuster, Simon K. and Fagerer, Stephan R. and Verboket, Pascal E. and Eyer, Klaus and Jefimovs, Konstantins and Zenobi, Renato and Dittrich, Petra S.",
  title = "{I}nterfacing droplet microfluidics with matrix-assisted laser desorption/ionization mass spectrometry: {L}abel-{F}ree {C}ontent {A}nalysis of {S}ingle {D}roplets",
  journal = "Analytical Chemistry",
  year = 2013,
  volume = "85",
  number = "3",
  pages = "1285--1289",
  month = feb,

E-Citations record created: Mon, 25 Mar 2013, 13:50:42 CET